SHC015V
MISSION® pLKO.1-puro-UbC-TagFP635™ Positive Control Transduction Particles
Contains a gene encoding TagFP635, under the control of the UbC promoter
Sinónimos:
MISSION®, MISSION® Control Transduction Particles
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
UNSPSC Code:
12352200
product line
MISSION®
technique(s)
capture ELISA: 106 TU/mL using p24
shipped in
dry ice
storage temp.
−70°C
¿Está buscando productos similares? Visita Guía de comparación de productos
General description
Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.
To see more application data, protocols, vector maps visit sigma.com/shrna.
Application
To see more application data, protocols, vector maps visit sigma.com/shrna.
When conducting experiments using MISSION shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. Silencing of the CMV promoter can be a problem in some cell types. For these cells, the ubiquitin promoter (UbC) can be a viable alternative. The MISSION UbC-TagFP635 Control Transduction Particles are a positive control to monitor transduction efficiency for applications in which an alternate promoter is necessary. The MISSION UbC-TagFP635 Control Transduction Particles are produced from the sequence-verified 9226 base pair lentivirus plasmid, pLKO.1-puro-UbC-TagFP635 (SHC015).
This construct aids in interpretation of experimental design and results by providing a far-red fluorescent protein marker for monitoring success in transduction of your cells of interest.
This construct aids in interpretation of experimental design and results by providing a far-red fluorescent protein marker for monitoring success in transduction of your cells of interest.
Legal Information
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TagFP635 is a trademark of Evrogen Co.
Optional
Referencia del producto
Descripción
Precios
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico