SAE0003

4-Hydroxyphenylpyruvate Dioxygenase from Streptomyces avermitilis

recombinant, expressed in E. coli

• Comisión internacional de enzimas: 1.13.11.27 (BRENDA, IUBMB)
• UNSPSC Code: 12352204
• NACRES: NA.54

Propiedades

• recombinant: expressed in E. coli
• Quality Level: 200
• form: liquid
• specific activity: ≥5 U/mg
• mol wt: ~41.8 kDa
• shipped in: dry ice
• storage temp.: −70°C

Descripción

General description

4-Hydroxyphenylpyruvate Dioxygenase (4-HPPD) is a key enzyme in the catabolism of tyrosine and phenylalanine. 4-HPPD catalyzes the synthesis of homogenistate (2,5-dihydroxyphenylacetate) from ρ-hydroxyphenylpyruvate and molecular oxygen. 4-HPPD also catalyzes the conversion of α-ketoisocaproate to Β-hydroxyisovalerate. 4-HPPD requires Fe²⁺ as an essential cofactor.

Rat 4-HPPD has a minimum of 80% homology with human 4-HPPD. Rat 4-HPPD is expressed abundantly in hepatocytes. It is also expressed in kidney, particularly in epithelial cells of the proximal tubules and the distant tubules. 4-HPPD has been identified as a potential biomarker of drug-induced liver injury in rat studies.

Unit Definition

One unit will oxidize 1 μmole of molecular oxygen per minute at pH 7.0, 25 °C, in the presence of 4-hydroxyphenylpyruvate.

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable