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Merck

RECOMT

Sigma-Aldrich

Thrombin CleanCleave Kit

Sinónimos:

Thrombin Cleavage Kit

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.56

form

suspension

Quality Level

mol wt

37 kDa

technique(s)

protein purification: suitable

shipped in

wet ice

storage temp.

2-8°C

General description

The Thrombin CleanCleave Kit contains a 50% suspension fo thrombin-agarose produced by immobilizing bovine thrombin and is designed for cleavage of recombinant fusion proteins

Application

For cleaving a tag from a recombinant fusion protein which contains the thrombin recognition sequence. Thrombin-agarose is compatible with recombinant proteins expressed in various types of expression systems.

Features and Benefits

  • Fast, efficient cleavage in as little as 2 hours
  • Thrombin is covalently bound to agarose for easy removal.
  • The robust cleavage reaction is effective at temperatures from 4 °C to 37 °C and over a wide range of pH and ionic strengths.
  • Cleave tags even in the presence of 0.1% Triton, 1 M urea or 5 mM EDTA
  • Thrombin-agarose is reusable.

Quantity

200 μl of a 50% slurry of thrombin-agarose cleaves >85% of 1 mg of fusion protein.

Legal Information

CleanCleave is a trademark of Sigma-Aldrich Co. LLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Solo componentes del kit

Referencia del producto
Descripción

  • Thrombin Cleavage Buffer, 10× 10 mL

Related product

Referencia del producto
Descripción
Precios

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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