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Merck
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RAB0377

Sigma-Aldrich

Visfatin EIA Kit

for serum, plasma, culture supernatant and cell lysates

Sinónimos:

Visfatin ELISA Kit

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

species reactivity

rat, human, mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
enzyme immunoassay: suitable

input

sample type serum
sample type culture supernatant(s)
sample type cell lysate
sample type plasma

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 0.778 ng/mL
standard curve range: 0.1-1000 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... NAMPT(10135)

General description

The Visfatin Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Visfatin peptide based on the principle of Competitive Enzyme Immunoassay.

Visfatin, also known as nicotinamide phosphoribosyltransferase (Nampt)/ pre-B Cell colony enhancing factor is encoded by the gene mapped to human chromosome 7q22.3. The gene contains 11 exons encompassing 34.7kb. The encoded protein is an adipocytokine secreted by visceral fat.

Immunogen

Visfatin, synthetic peptide

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Visfatin EIA Kit has been used to measure the concentration of visfatin in blood samples.

Biochem/physiol Actions

Visfatin has a role in mammalian nicotinamide adenine dinucleotide (NAD+) biosynthesis. Studies have shown that mice deficient in visfatin in the forebrain excitatory neurons show hyperactivity, diminished anxiety-like behaviors and impaired learning and memory. It has also been shown that visfatin stimulates glucose uptake and adenosine monophosphate-activated protein kinase (AMPK) phosphorylation in mouse C2C12 skeletal muscle cells. Visfatin regulates inflammation and insulin sensitivity.

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS

pictograms

Corrosion
GHS05

signalword

Warning

hcodes

H290

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible corrosive hazardous materials

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

Single and Concurrent Effects of Endurance and Resistance Training on Plasma Visfatin, Insulin, Glucose and Insulin Resistance of Non-Athlete Men with Obesity.
Tayebi S M, et al.
Annals of applied sport science., 4(4), 21-31 (2016)
Cahit Birdir et al.
The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians, 30(6), 629-634 (2016-04-29)
Investigating potential value of maternal serum Visfatin, sFlt-1, PlGF, AFP, PAPP-A levels at first trimester for prediction of small for gestational age (SGA) at birth. Measurements were performed in 20 SGA and 65 control cases. Logistic regression analysis adjusted for
Effects of Diet and Exercise-Induced Weight Loss in Sedentary Obese Women on Inflammatory Markers, Resistin, and Visfatin.
Khanna D, et al.
Obesity and Nutrition of J, 102 (2017)
Matthaios G Pitoulias et al.
Cytokine, 91, 140-144 (2017-01-11)
Visfatin is an adipokine molecule acting as an essential coenzyme in multiple cellular redox reactions. The increased serum levels of Visfatin have been correlated with metabolic syndrome and endothelial homeostasis. In this study we investigate the possible relationship of Visfatin
A visfatin promoter polymorphism is associated with low?grade inflammation and type 2 diabetes.
Zhang Y-Y, et al.
Obesity (Silver Spring, Md.), 14(12), 2119-2126 (2006)
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