P6911
Proteasa from Streptomyces griseus
BioReagent, DNase, RNase, and nickase, none detected (No RNase.)
Sinónimos:
Actinasa E, Pronasa E
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Número de CAS:
EC Number:
MDL number:
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.21
Productos recomendados
grade
for molecular biology
Quality Level
product line
BioReagent
form
powder
mol wt
monomer ~20 kDa
concentration
≥4 unit/mg
solubility
water: 5-20 mg/mL
foreign activity
DNase, RNase, and nickase, none detected (No RNase.)
storage temp.
−20°C
¿Está buscando productos similares? Visita Guía de comparación de productos
General description
A mixture of at least three proteolytic activities including an extracellular serine protease. In general, serine proteases display a wide range of substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule. This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid.
Specificity
Mezcla de como mínimo tres actividades proteolíticas, entre ellas una serín proteasa extracelular. En general, las proteasas de serina exhiben una amplia variedad de especificidades de sustrato, que se creen mediadas por un centro activo compuesto de un resto de Asp, uno de His y uno de Ser en la molécula. Esta enzima prefiere hidrolizar los enlaces peptídicos del lado carboxilo de los ácidos glutámico o aspártico.
Application
Esta enzima es más activa a un intervalo de pH más elevado que la proteasa alcalina conocida, mostrando actividad proteolítica incluso en disolución de NaOH 0,2 N. Esta enzima es útil para la proteólisis de proteínas insolubles y para la investigación de la estructura de las proteínas.
La proteasa se utiliza normalmente en los procedimientos de aislamiento de ácidos nucleicos en incubaciones de 0,5 a 3,0 horas complementadas con dodecilsulfatosódico al 0,2 % y EDTA 10mM.
Suitable for:
- Nucleic acid isolation procedures in incubations
- Degrade protein during nucleic acid purification
- Proteolysis of insoluble protein
- Structural protein studies
Physical properties
Completamente inactivada al calentarse por encima de 80 °C durante 15 a 20 minutos.
Unit Definition
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
Preparation Note
Recolectada del caldo de cultivo de S. griseus.
Analysis Note
The protease is incubated for 10 minutes at pH 7.5 at 37°C in a 6 ml reaction volume containing 0.54% casein and 0.041 M potassium phosphate buffer. The reaction is stopped by the addition of 5.0 ml of 0.11 M trichloroacetic acid.
Other Notes
This protease is completely inactivated by heating above 80°C for 15-20 minutes. This enzyme is more active at a higher pH range, showing the proteolytic activity even in 0.2N NaOH solution.
Related product
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
D Y Yum et al.
Bioscience, biotechnology, and biochemistry, 58(3), 470-474 (1994-03-01)
SAP, an extracellular alkaline serine protease produced by Streptomyces sp. YSA-130, was purified to homogeneity by CM-Sephadex column chromatography and crystallization. The enzyme was a monomeric protein with a molecular weight of 19,000 as estimated by SDS-PAGE and gel filtration.
N Yoshida et al.
Journal of biochemistry, 104(3), 451-456 (1988-09-01)
A protease was purified 163-fold from Pronase, a commercial product from culture filtrate of Streptomyces griseus, by a series of column chromatographies on CM-Toyopearl (Fractogel), Sephadex G-50, hydroxyapatite, and Z-Gly-D-Phe-AH-Sepharose 4B using Boc-Ala-Ala-Pro-Glu-pNA as a substrate. The final preparation was
Hyun-Ju Cho et al.
Disease models & mechanisms, 12(5) (2019-05-03)
DYRK1A is a major causative gene in Down syndrome (DS). Reduced incidence of solid tumors such as neuroblastoma in DS patients and increased vascular anomalies in DS fetuses suggest a potential role of DYRK1A in angiogenic processes, but in vivo
P Bressollier et al.
Applied and environmental microbiology, 65(6), 2570-2576 (1999-05-29)
Streptomyces strain K1-02, which was identified as a strain of Streptomyces albidoflavus, secreted at least six extracellular proteases when it was cultured on feather meal-based medium. The major keratinolytic serine proteinase was purified to homogeneity by a two-step procedure. This
Aline C Habison et al.
PLoS pathogens, 13(9), e1006555-e1006555 (2017-09-15)
Many pathogens, including Kaposi's sarcoma herpesvirus (KSHV), lack tractable small animal models. KSHV persists as a multi-copy, nuclear episome in latently infected cells. KSHV latency-associated nuclear antigen (kLANA) binds viral terminal repeat (kTR) DNA to mediate episome persistence. Model pathogen
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico