P350-05

Porcine Coronary Artery Smooth Muscle Cells: PCASMC (Cryovial)

• UNSPSC Code: 41106514
• NACRES: NA.81

Propiedades

• biological source: Porcine coronary artery
• Quality Level: 100
• form: solid
• packaging: pkg of 500,000 cells
• manufacturer/tradename: Cell Applications, Inc
• growth mode: Adherent
• karyotype: 2n = 38
• morphology: smooth muscle
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): diabetes; stroke; cardiovascular diseases
• shipped in: dry ice
• storage temp.: −196°C

Descripción

General description

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PCASMC were used to investigate the role of c-Jun in smooth muscle proliferation, and to show that c-Jun inhibition abrogates smooth muscle cell growth and intimal thickening after arterial injury (Khachigian, 2002). PCASMC were also used to develop poly (l-lactide-co-caprolactone) nanofibrous scaffold material with potential for tissue engineering (Dong, 2008).

Characterization: positive for smooth muscle cell specific alpha-actin expression.

Cell Line Origin

Artery

Application

vascular research, supply of blood to heart muscle

Components

Porcine Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO

Preparation Note

• 2nd passage, >500,000 cells in Porcine Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO
• Can be cultured at least 16 doublings

Subculture Routine

Please refer to the PCASMC Culture Protocol.

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable