N4645

Fosfato de 4-nitrofenilo disodium salt hexahydrate

powder, BioReagent, suitable for cell culture, ≥97%

• Sinónimos: 4-Nitrofenil fosfato de disodio, 4-Nitrofenil fosfato de sodio hexahydrate, Fosfato de p-nitrofenilo disodium hexahydrate, pNPP disodium hexahydrate
• Fórmula lineal: O₂NC₆H₄OP(O)(ONa)₂ · 6H₂O
• Número de CAS: 333338-18-4
• Peso molecular: 371.14
• Beilstein/REAXYS Number: 5324661
• EC Number: 224-246-5
• MDL number: MFCD00066288
• UNSPSC Code: 12352204
• PubChem Substance ID: 24897708
• NACRES: NA.75

Propiedades

• biological source: synthetic (organic)
• Quality Level: 200
• product line: BioReagent
• assay: ≥97%
• form: powder
• technique(s): cell culture | mammalian: suitable
• solubility: H₂O: 100 mg/mL
• storage temp.: −20°C
• SMILES string: O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
• InChI: 1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
• InChI key: KAKKHKRHCKCAGH-UHFFFAOYSA-L

Descripción

General description

Sustrato cromógeno para la determinación de fosfatasas ácidas y alcalinas. La reacción da un producto final soluble que es de color amarillo. La reacción puede detenerse con NaOH 3 N y leerse a 405 nm.

Application

4-Nitrophenyl phosphate disodium salt hexahydrate has been used:

• as a component in diethanolamine buffer to detect alkaline phosphatase-conjugated antibodies in enzyme linked immunosorbent assay (ELISA)
• to treat adherent cells in adhesion assay
• as a phosphatase substrate in in vitro phosphatase assay in substrate buffer for ELISA

Biochem/physiol Actions

p-Nitrophenyl phosphate (pNPP) is the substrate of choice for use with alkaline phosphatase in enzyme linked immunosorbent assay (ELISA) procedures. It is the substrate of choice in alkaline phosphatase systems due to its high sensitivity. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. This product is tested for use in cell culture applications. 4-nitrophenyl phosphate (pNPP), a small-molecule substrate can be used to study on alkaline phosphatases, acid phosphatases etc.

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)