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Merck

MAK190

Sigma-Aldrich

ATP Assay Kit

Sufficient for 100 Colorimetric or Fluorometric tests

Sinónimos:

Adenosine triphosphate Assay Kit

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About This Item

EC Number:
UNSPSC Code:
12161503
NACRES:
NA.84

detection method

colorimetric
fluorometric

relevant disease(s)

cancer; neurological disorders

storage temp.

−20°C

General description

Adenosine triphosphate (ATP) is a nucleoside triphosphate formed exclusively in the mitochondria. It is a high-energy molecule described as the energy currency in all living systems. The chemical energy contained in the phosphate bond of ATP drives most cellular processes. Genetic disorders such as Leber hereditary optic neuropathy, Leigh syndrome, neuropathy, ataxia, and retinitis pigmentosa affect the generation of ATP in the mitochondria.

Application

ATP Assay Kit has been used to quantify the ATP concentration by fluorometric detection of ATP in samples.

Suitability

Suitable for the determination of ATP in samples such as animal tissue and cell culture samples.

Principle

The ATP Colorimetric/Fluorometric Assay Kit is a sensitive assay for determining ATP in various samples (ranging from 2–10 nmole/well for the colorimetric assay and from 20–1,000 pmole/well for the fluorometric assay). ATP concentration is determined by phosphorylating glycerol, resulting in a colorimetric (570 nm) or fluorometric (lex = 535/lem = 587 nm) product proportional to the amount of ATP present.

Other Notes

The fluorometric assay is 10-100 times more sensitive than the colorimetric assay.

Solo componentes del kit

Referencia del producto
Descripción

  • ATP Assay Buffer

  • ATP Probe, in DMSO

  • ATP Converter

  • Developer Mix

  • ATP Standard, 1.0 μmole

Replaced by

Referencia del producto
Descripción
Precios

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

flash_point_f

188.6 °F

flash_point_c

87 °C


Certificados de análisis (COA)

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