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Merck

MAK077

Sigma-Aldrich

Uric Acid Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric
fluorometric

relevant disease(s)

endocrinological disorders, diabetes; cardiovascular diseases; rheumatological diseases; genitourinary disorders

storage temp.

−20°C

General description

In humans and primates, uric acid is the end product of purine metabolism. It is produced by the oxidation of xanthine and hypoxanthine by xanthine oxidase and excreted in urine. High serum levels of uric acid, hyperuricemia, are associated with insulin resistance, cardiovascular disease, and gout. The mechanisms leading to hyperuricemia are typically either increased uric acid production or decreased urine excretion. Increased serum uric acid may be a marker of renal disease.

Application

Uric Acid Assay Kit has been used
  • to determine the uric acid content in human adipose-derived stem cell culture supernatant
  • to measure serum uric acid level in order to analyse kidney function in rat models
  • to determine the uric acid concentration in serum and to study the e antiinflammatory effects of sunflower head ethanol extract in rats
  • in quantitative analysis of uric acid degradation

Suitability

Suitable for detecting uric acid in biological samples such as serum and urine. Pretreatment of samples is not required

Principle

In this assay, Uric Acid concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the Uric Acid present.

related product

Referencia del producto
Descripción
Precios

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Aquatic Chronic 3 - Resp. Sens. 1

Storage Class

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup


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Stephanie A Terry et al.
Journal of animal science, 96(9), 3863-3877 (2018-09-01)
Ruminants play an important role in food security, but there is a growing concern about the impact of cattle on the environment, particularly regarding greenhouse gas emissions. The objective of this study was to examine the effect of humic substances
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Frontiers in veterinary science, 6, 308-308 (2019-10-15)
The objective of this study was to examine the effect of a pine enhanced biochar (EB) on rumen fermentation, apparent total tract digestibility, methane (CH4) emissions, and the rumen and fecal microbiome of Angus × Hereford heifers fed a barley
A Role for Uric Acid in the Progression of Renal Disease.
Kang, D.-H
Journal of the American Society of Nephrology, 12, 2888-2897 (2002)
Anti-gouty arthritis and antihyperuricemia effects of sunflower (Helianthus annuus) head extract in gouty and hyperuricemia animal models.
Li L, et al.
BioMed Research International, 2017 (2017)
Effect of humic substances on rumen fermentation, nutrient digestibility, methane emissions, and rumen microbiota in beef heifers.
Terry S A, et al.
Journal of Animal Science (2018)

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

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