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Key Documents

L9641

Sigma-Aldrich

Lipopolysaccharides (rough strains) from Escherichia coli EH100 (Ra mutant)

Sinónimos:

LPS

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About This Item

EC Number:
MDL number:
UNSPSC Code:
12352201
NACRES:
NA.25

biological source

Escherichia coli (EH100 Ra mutant)

Quality Level

form

lyophilized powder

impurities

<3% Protein (Lowry)

color

white to yellow

solubility

water: 4.90-5.10 mg/mL, cloudy to turbid, colorless to faintly yellow

shipped in

ambient

storage temp.

2-8°C

General description

This product is phenol:chloroform:petroleum ether extracted from E. coli EH 100 (Ra mutant). The source strain is from a private collection.

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

Prepared by phenol-chloroform-petroleum ether extraction.

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

pictograms

Skull and crossbones

signalword

Danger

hcodes

Hazard Classifications

Acute Tox. 2 Oral

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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