L8262
Lectin from Ulex europaeus (gorse, furze)
biotin conjugate, lyophilized powder
Sinónimos:
Ulex europaeus agglutinin, UEA-I
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
Productos recomendados
conjugate
biotin conjugate
Quality Level
form
lyophilized powder
potency
<50 μg/mL agglutination activity
composition
Protein, ~85% E1%/280
extent of labeling
~3 mol biotin per mol protein
storage temp.
2-8°C
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Application
Lectin from Ulex europaeus (gorse, furze) has been used:
- as a Paneth cells marker in immunohistochemistry at 1:1,000 dilution
- in double staining for endothelial cells for quantitation of blood vessels
- in histochemical staining of bronchial epithelial cells
Biochem/physiol Actions
Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.
Packaging
Package size based on protein content.
Linkage
Conjugates are prepared from affinity purified lectin.
Physical form
Contains sodium citrate
Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Daisuke Kondoh et al.
Cells, tissues, organs, 203(1), 20-28 (2016-07-18)
Glycoconjugates in the olfactory system play critical roles in neuronal formation, and α1-2 fucose (α1-2Fuc) glycan mediates neurite outgrowth and synaptic plasticity. Histochemical findings of α1-2Fuc glycan in the mouse olfactory system detected using Ulex europaeus agglutinin-I (UEA-I) vary. This
M Tango et al.
Archives of histology and cytology, 63(1), 81-89 (2000-04-19)
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