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L6544

Sigma-Aldrich

LuminoCt® SYBR® Green qPCR ReadyMix™

For fast SYBR Green quantitative PCR

Sinónimos:

LuminoCt®

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About This Item

UNSPSC Code:
41106300
NACRES:
NA.55

form

liquid

usage

sufficient for 100 reactions
 sufficient for 2000 reactions
 sufficient for 500 reactions

feature

dNTPs included
hotstart

technique(s)

qPCR: suitable

color

colorless

input

purified DNA

application(s)

agriculture

compatibility

Bio-Rad MyiQ
 Bio-Rad iCycler iQ
 Bio-Rad iQ 5
for use with ABI 5700
for use with ABI 7000
for use with ABI 7300
for use with ABI 7500 Fast
for use with ABI 7500
for use with ABI 7700
for use with ABI 7900 Fast
for use with ABI 7900 HT
for use with ABI 7900
for use with ABI StepOne
for use with ABI StepOnePlus
for use with ABI ViiA 7
for use with Bio-Rad CFX384
for use with Bio-Rad CFX96
for use with Bio-Rad MJ Chromo4
for use with Bio-Rad MJ Opticon 2
for use with Bio-Rad MJ Opticon Cepheid SmartCycler
for use with Bio-Rad MJ Opticon
for use with Bio-Rad MiniOpticon
for use with Eppendorf® Mastercycler ep realplex2 s
for use with Eppendorf® Mastercycler ep realplex
for use with Illumina Eco qPCR
for use with Qiagen Corbett Rotor-Gene 3000
for use with Qiagen Corbett Rotor-Gene 6000
for use with Qiagen Corbett Rotor-Gene Q
for use with Roche LightCycler 480
for use with Strategene Mx3000P
for use with Strategene Mx3005P
for use with Strategene Mx4000

detection method

SYBR® Green

shipped in

wet ice

storage temp.

−20°C

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General description

LuminoCt® SYBR® Green qPCR ReadyMix is ready to use master mix that combines the performance enhancements of our JumpStart™ Taq antibody for hot start. Extensive design and validation of the ReadyMix chemistry has virtually eliminated the need for optimization when LuminoCt is used in conjunction with properly designed primers. Inclusion of JumpStart™ Taq antibody in the ReadyMix eliminates polymerase activity at ambient temperatures without causing the performance issues associated with chemically modified hot-start Taq. This allows for very rapid activation of the Taq and delivers unparalleled assay sensitivity, while allowing for benchtop reaction setup.
  • Kit is designed to perform SYBR Green-based qPCR assays on commonly available real-time instrument platforms
  • ReadyMix requires the addition of reference dye (provided in kit) when being used on real-time instruments that require ROX passive reference dye for normalization of qPCR assays
  • Kit is not compatible with qPCR instruments that utilize glass capillary tubes

Application

LuminoCt® SYBR® Green qPCR ReadyMix™ has been used for fast quantitative polymerase chain reaction (qPCR) and quantitative RT-PCR amplifications. It has also been used in qPCR for measuring gene expression.

Features and Benefits

  • Ideal for performing high-throughput, quantitative PCR applications
  • The master mix allows consistency from one reaction to the next
  • Reduced preparation time and reduced risk of contamination from multiple pipetting steps
  • The hot start mechanism using the JumpStart Taq antibody prevents amplification of non-specific products, resulting in increased efficiency and higher target yield
  • LuminoCt® ReadyMixes require the use of small amplicons (<200bp) for optimal results
  • This master mix is compatible with commercial primer sets, including TaqMan® assays
  • Assay results in as little as 25 minutes
  • Unsurpassed accuracy, precision, and sensitivity
  • Virtually eliminates the need to optimize assay parameters

Packaging

Default reaction volume is 50 μL

100RXN is packaged as 1 X 2.5 mL
500RXN is packaged as 1 X 12.5 mL
2000RXN is packaged as 1 X 50 mL

Other Notes

  • At a minimum, use software to design primers for assays and ensure that qPCR amplicons are <200 bp
  • Sigma recommends designing primers and probes with Beacon Designer software
  • To order primers and probes from Sigma, visit: www.sigma.com/oligos

Legal Information

Eppendorf is a registered trademark of Eppendorf AG
JumpStart is a trademark of Sigma-Aldrich Co. LLC
LuminoCt is a registered trademark of Merck KGaA, Darmstadt, Germany
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies
SabreLite is a registered trademark of Pelican Products, Inc.
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Solo componentes del kit

Referencia del producto
Descripción
  • 2 X

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS
  • R4526100X ROX internal reference dyeSDS

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KiCqStart® SYBR® Green qPCR ReadyMix™, For Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems

KCQS01

KiCqStart® SYBR® Green qPCR ReadyMix™, Low ROX™, for ABI and Stratagene instruments

KCQS02

KiCqStart® SYBR® Green qPCR ReadyMix™, with ROX™ for ABI instruments

KCQS03

KiCqStart® SYBR® Green qPCR ReadyMix™, iQ™, with fluorescein for Bio-Rad systems

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Studies of vulval development in the nematode C. elegans have identified many genes that are involved in cell division and differentiation processes. Some of these encode components of conserved signal transduction pathways mediated by EGF, Notch, and Wnt. To understand
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