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L4025

Sigma-Aldrich

Lyticase from Arthrobacter luteus

lyophilized powder, ≥200 units/mg solid

Sinónimos:

(1,3)-β-D-Glucan endohydrolase, 1,3-β-Glucan glucohydrolase, Bacterial lyticase, Lysing enzyme

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About This Item

Número de CAS:
37340-57-1
MDL number:
MFCD00131560
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bacterial (Arthrobacter luteus)

Quality Level

200

form

lyophilized powder

specific activity

≥200 units/mg solid

technique(s)

cell based assay: suitable

suitability

suitable for cell lysis

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

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Application

Lyticase from Arthrobacter luteus has been used for resuspending cells to extract high-molecular weight DNA for polymerase chain reaction (PCR) fingerprint analyses.
Lyticase from Arthrobacter luteus has been used:
  • as a component in spheroplasting buffer to prepare yeast nuclear extracts
  • for digestion in sample preparation for β-glucan enzymatic assay using yeast
  • to lyse cells for RNA isolation

Biochem/physiol Actions

Lyticase enzyme is frequently used in fungal research, particularly for species identification using polymerase chain reaction (PCR)-based techniques. It can break down β (1→3) and β (1→4) bonds between glucose units.
Lyticase is a lysing enzyme that is used to extract DNA from yeast cells by inducing partial spheroplast formation. Spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast, which are difficult to disrupt because the cell walls may form capsules or resistant spores. Lyticase contains β-(1→;3)-glucan laminaripentaohydrolase along with β-(1→3)-glucanase, protease, and mannanase activities. It is useful with yeast cells such as e.g. Candida, Debaryomyces, Saccharomyces, Saccharomycopsis, Saccharomycodes, Eremothecium, and Schwanniomyces species.
Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.

Unit Definition

One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Protocolos

Enzymatic Assay of Lyticase

This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate.

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