L2769

Lectina de Phaseolus vulgaris (frijol rojo)

Leucoagglutinin PHA-L, lyophilized powder

• Sinónimos: Aglutinina de Phaseolus vulgaris, PHA
• UNSPSC Code: 12352202
• NACRES: NA.32

Propiedades

• biological source: Phaseolus vulgaris
• form: lyophilized powder
• potency: erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at <62.5 μg lectin/ml.); ≤1.0 μg/mL leucoagglutination activity; ≤5 μg/mL mitogenic activity
• composition: Protein, ≥60% biuret
• impurities: salt, free
• storage temp.: 2-8°C

Descripción

General description

Phytohemagglutinin (PHA) derived from Phaseolus vulgaris is a tetrameric glycoprotein. It consists of two different subunits, that weighs 120 kDa.

Application

Lectin from Phaseolus vulgaris (red kidney bean) has been used:

• as a positive control in ELISPOT (enzyme-linked immunospot) assay
• to induce Th2 polarization for T-cell differentiation in vitro
• as controls in the lymphocyte proliferation assays

Biochem/physiol Actions

La PHA consta de dos especies moleculares, la eritroaglutinina (PHA-E), que tiene poca actividad mitógena y elevada actividad de eritroaglutinina, y la leoucoaglutinina (PHA-L), que tiene elevada actividad mitógena y leoucoaglutinante, pero muy baja actividad eritroaglutininante
La PHA-E no es específica de grupo sanguíneo, pero la aglutinación puede ser inhibida por ciertos oligosacáridos. La PHA-P es la forma proteica de la PHA antes de la separación y la purificación de la eritroaglutinina y la leoucoaglutinina. La PHA-M es la forma mucoproteínica. Los conjugados se preparan a partir de las correspondientes lectinas purificadas.

Preparation Note

Purified by a modification of the method of Leavitt.

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of either human erythrocytes (2% in phosphate buffered saline, pH 6.8) or human leukocytes (10⁷ per mL in saline) after 1 hr incubation at 25 °C.

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)