L1378
L-Lactic Dehydrogenase from bovine muscle
Type X, ammonium sulfate suspension, ≥600 units/mg protein
Sinónimos:
(S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD, Lactate
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Productos recomendados
biological source
bovine muscle
Quality Level
type
Type X
assay
3.0—25.0 mg protein/mL (biuret)
form
ammonium sulfate suspension
specific activity
≥600 units/mg protein
manufacturer/tradename
Sigma-Aldrich
technique(s)
activity assay: suitable
color
white
pH
7.5
application(s)
life science and biopharma
foreign activity
pyruvate kinase ≤0.01%
storage temp.
2-8°C
Gene Information
cow ... LDHA(281274) , LDHB(281275)
General description
Research Area: Cell Signaling
Lactate dehydrogenase (LDH) is a key enzyme in the anaerobic metabolic pathway and falls under the class of oxidoreductases. This enzyme is found in a wide range of organisms, including both plants and animals. It is widely distributed across all tissues. LDH is primarily a cytoplasmic enzyme, with particularly high concentrations in muscle, liver, and kidney tissues.
Lactate dehydrogenase (LDH) is a key enzyme in the anaerobic metabolic pathway and falls under the class of oxidoreductases. This enzyme is found in a wide range of organisms, including both plants and animals. It is widely distributed across all tissues. LDH is primarily a cytoplasmic enzyme, with particularly high concentrations in muscle, liver, and kidney tissues.
Application
L-Lactic Dehydrogenase from bovine muscle has been used in lactate assay to assess lactate tissue accumulation.
Biochem/physiol Actions
Lactate dehydrogenase (LDH) plays a critical role as a checkpoint in gluconeogenesis and DNA metabolism. It plays a vital role in maintaining homeostasis during periods of low oxygen availability. As an oxidoreductase enzyme, lactate dehydrogenase catalyzes the reversible conversion of pyruvate to lactate using NADH. This enzyme is crucial for the anaerobic metabolism of glucose when oxygen is either absent or in short supply. LDH is the sole serum biomarker effective for evaluating metastatic melanomas.
Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.
Unit Definition
One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.
Physical form
Crystalline suspension in 2.4 M (NH4)2SO4 solution, pH 6.0
Analysis Note
Protein determined by biuret.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
ppe
Eyeshields, Gloves, type N95 (US)
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Vasco Meneghini et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(14), 6047-6059 (2013-04-05)
Dysregulated hippocampal neurogenesis has been associated with neurodegenerative disorders, including Alzheimer's disease (AD), in which it may potentially represent an auto-reparatory mechanism that could counteract neuronal loss and cognitive impairment. We evaluated hippocampal neurogenesis in TgCRND8 mice and reported that
Sebastian-Jonas Saur et al.
Deutsche medizinische Wochenschrift (1946), 138(16), Seite 1-Seite30 (2013-04-24)
We report on the case of a young women presenting with macrohaematuria, petechiae and strong headaches. Laboratory showed a thrombotic microangiopathy with helmet cells, increased LDH levels (>600 U/l), and thrombocytopenia (<40,000/μl). Due to strong haemolytic activity and headache with
Wei Quan et al.
Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan, 33(1), 85-91 (2013-04-20)
To determine the cardioprotective effect of magnesium lithospermate B (MLB) on myocardial ischemia/reperfusion (MI/R) injury and to investigate the antioxidant potential in vivo and in vitro. MI/R injury was induced by the occlusion of left anterior descending coronary artery for
O V Basharina et al.
Radiatsionnaia biologiia, radioecologiia, 52(6), 602-607 (2013-03-23)
The influence of UV-light (240-390 nm) at the dozes of 151 and 755 J/m2 on the intensity of processes of the lipid peroxidation, activity of lactate dehydrogenase (LDH), succinate dehydrogenase (SDH), cytochrome c oxidase and the level of the energy
Bécaye Fall et al.
Malaria journal, 12, 107-107 (2013-03-21)
In 2006, the Senegalese National Malaria Control Programme recommended artemisinin-based combination therapy (ACT) as the first-line treatment for uncomplicated malaria. Since the introduction of ACT, there have been very few reports on the level of resistance of P. falciparum to
Artículos
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico