I8782
Interferon αA from mouse
≥95%, recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture
Sinónimos:
IFN-αA
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About This Item
Productos recomendados
biological source
mouse
Quality Level
recombinant
expressed in E. coli
assay
≥95%
form
buffered aqueous solution
mol wt
20 kDa
technique(s)
cell culture | mammalian: suitable
color
colorless
shipped in
dry ice
storage temp.
−70°C
Gene Information
mouse ... Ifna3(110218)
Biochem/physiol Actions
Cytokine with anti-viral, anti-proliferative, immunoregulatory, and proinflammatory activities. Type I Interferons are a closely related family of 165-172 amino acid proteins that are produced by leukocytes (α subtypes), fibroblasts (β subtypes), lymphocytes (ω subtypes) and ruminant embryos (τ subtypes). Interferon induces a variety of effects on target cells including antiviral, antiproliferative and immunomodulatory activities.
Packaging
Minimum 1x105 units/vial.
Physical form
Solution in phosphate buffered saline containing 0.1% bovine serum albumin.
Analysis Note
The biological activity is determined in the cytopathic effect inhibition assay using mouse L929 cells with encephalomyocarditis virus. The cytopathic effect of 50% is produced with approximately 1 unit/ml interferon. The units are determined with respect to NIH international standard reference for mouse interferon α.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Interferon standards and general abbreviations.
S Pestka
Methods in enzymology, 119, 14-23 (1986-01-01)
A convenient and rapid cytopathic effect inhibition assay for interferon.
P C Familletti et al.
Methods in enzymology, 78(Pt A), 387-394 (1981-01-01)
S Rubinstein et al.
Journal of virology, 37(2), 755-758 (1981-02-01)
A convenient assay for interferons based on reduction of cytopathic effect was developed. The number of manipulations and the lengths of the various incubation steps were reduced to a minimum. The assay is simple to perform and can be completed
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