I4640
Human IgG4 Kappa, Recombinant Protein
purified immunoglobulin, buffered aqueous solution
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About This Item
Productos recomendados
biological source
human
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
assay
>95% (SDS-PAGE)
form
buffered aqueous solution
shipped in
dry ice
storage temp.
−20°C
General description
IgG antibody subtype is the most abundant serum immunoglobulin of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to the fetus through the placenta and is vital for immune defense of the neonate against infections. IgG4, κ recombinant protein is obtained by expression of a phage library in a human cell line and purified by affinity chromatography.
Application
The purified IgG4, κ may be used as an immunoglobulin calibrator, reference antigen, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot blot immunoblotting, Western immunoblotting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell-binding assays.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4.
Analysis Note
The purity and identity are determined by immunoelectrophoresis, indirect ELISA, and SDS-PAGE.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
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Journal of proteome research, 21(1), 274-288 (2021-12-09)
Methods of antibody detection are used to assess exposure or immunity to a pathogen. Here, we present Ig-MS, a novel serological readout that captures the immunoglobulin (Ig) repertoire at molecular resolution, including entire variable regions in Ig light and heavy
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