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I4510

Sigma-Aldrich

Iminodiacetic acid Sepharose™

aqueous ethanol suspension

Sinónimos:

Iminodiacetic acid Agarose, IDA Resin

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About This Item

MDL number:
MFCD00801334
UNSPSC Code:
23151817
PubChem Substance ID:
329815374
NACRES:
NA.56

form

aqueous ethanol suspension

matrix

Sepharose 6B Fast Flow

matrix activation

epoxy

matrix attachment

amino

matrix spacer

7 atoms

capacity

22-30 μmol/mL binding capacity (Zn2+)

storage temp.

2-8°C

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General description

I4510-50ML′s updated product number is GE17-0575-01
Iminodiacetic acid Sepharose™ is a chelating resin used for the purification of proteins.

Application

Iminodiacetic acid Sepharose™ has been slurry-packed into a tared polypropylene column for immobilized metal-ion affinity chromatography (IMAC) and for the preparation of the chelating sepharose column. It is an important component of metal affinity chromatography which immobilizes transition metal ions.
Iminodiacetic acid Sepharose™ is used is affinity chromatography, protein chromatography and chelating resins. Iminodiacetic acid Sepharose™ has been used to report a new procedure for the purification of two human monoclonal anti-HIV (human immunodeficiency virus) antibodies (mAbs 2G12 and 4E10).

Physical form

Suspension in 20% ethanol

Legal Information

Sepharose is a trademark of Cytiva

Related product

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pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk_germany

WGK 1

flash_point_f

96.8 °F

flash_point_c

36 °C

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Histidine-rich glycoprotein from the hemolymph of the marine mussel Mytilus edulis L. binds Class A, Class B, and borderline metals
Devoid SJ, et al.
Environmental Toxicology and Chemistry / Setac, 26(5), 872-877 (2007)
Identification and quantification of histidine-rich glycoprotein (HRG) in the blood plasma of six marine bivalves.
Abebe, A.T., et al.
Comp. Biochem. Physiol., B: Comp. Biochem., 147(1), 74-81 (2007)
B Sutter et al.
Protein expression and purification, 19(1), 53-56 (2000-06-02)
The superoxide dismutases (EC 1.15.1.1) are a family of enzymes that catalyze the dismutation of superoxide radical anion to dioxygen and hydrogen peroxide. The active site contains a critical metal ion such as manganese, iron, or copper. The copper-containing protein
Immobilized iminodiacetic acid metal peptide complexes. Identification of chelating peptide purification handles for recombinant proteins
Smith MC, et al.
Inorganic Chemistry, 26(12), 1965-1969 (1987)
A Naeem et al.
Biochemistry. Biokhimiia, 71(1), 56-59 (2006-02-07)
Concanavalin A and a mannose-specific lectin could be precipitated specifically from extracts of jack bean and Cajanus cajan seeds, respectively, using metal charged EGTA. Single step purification of the lectins was also possible using iminodiacetic acid-Sepharose charged with metal ions.
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