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Merck

I1146

Sigma-Aldrich

R3 IGF-1 human

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Sinónimos:

Human IGF-1, IGF-1 Protein, Recombinant IGF-1

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About This Item

UNSPSC Code:
51111800
NACRES:
NA.77

biological source

human

Quality Level

recombinant

expressed in E. coli

assay

≥85% (HPLC)

form

lyophilized powder

potency

≤10 ng/mL ED50

quality

endotoxin tested

mol wt

7,676 Da

packaging

pkg of 0.1 mg

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

storage temp.

2-8°C

Gene Information

human ... IGF1(3479)

Application

R3 IGF-I is a recombinant analog of insulin-like growth factor containing the complete human IGF-I amino acid sequence with substitution of Arg for Glu3. It was developed as an inexpensive, high quality potent analog of IGF for use as a growth factor supplement for serum-free or low serum cell culture.

Recombinantly produced in E. coli using a patented expression system, the peptide is harvested from cells in inclusion bodies, which are dissolved and desalted. The active molecule is properly folded under oxidizing conditions and then purified by several liquid chromatography steps.

Biochem/physiol Actions

Proliferation of chinese hamster ovary CHO cells.

Other Notes

Recombinant analog of insulin-like growth factor-I with the substitution of Arg for Glu at position 3.

Preparation Note

Prepared from 0.1 M acetic acid

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Yuki Kiyokawa et al.
Journal of clinical medicine, 9(9) (2020-09-06)
Pluripotent stem cells are classified as naïve and primed cells, based on their in vitro growth characteristics and potential to differentiate into various types of cells. Human-induced pluripotent stem cells (iPSCs, also known as epiblast stem cells [EpiSCs]) have limited
Peter Tontonoz et al.
Molecular and cellular biology, 35(7), 1125-1138 (2015-01-22)
We previously showed that the orphan nuclear receptor Nur77 (Nr4a1) plays an important role in the regulation of glucose homeostasis and oxidative metabolism in skeletal muscle. Here, we show using both gain- and loss-of-function models that Nur77 is also a

Artículos

Insulin-like Growth Factors (IGF)

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