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H5000

Sigma-Aldrich

Hexokinase from Saccharomyces cerevisiae

Type III, lyophilized powder, ≥25 units/mg protein (biuret)

Sinónimos:

ATP:D-Hexose-6-phosphotransferase, Hexokinase from yeast

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About This Item

Número de CAS:
9001-51-8
Comisión internacional de enzimas:
2.7.1.1 (BRENDA, IUBMB)
EC Number:
232-611-5
MDL number:
MFCD00131321
UNSPSC Code:
12352204
eCl@ss:
32160410

type

Type III

form

lyophilized powder

specific activity

≥25 units/mg protein (biuret)

mol wt

dimer 110 kDa

UniProt accession no.

P04806
P04807

foreign activity

glucose-6-phosphate dehydrogenase and phosphoglucose isomerase ≤10%

storage temp.

−20°C

Gene Information

bakers yeast ... HXK1(850614) , HXK2(852639)

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Biochem/physiol Actions

Catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing ATP as a phosphate source.

The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C).
D-fructose KM: 0.33 mM
D-glucose KM: 0.12 mM
D-mannose KM: 0.05 mM

Yeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively.

Molecular Weight: ~ 54 kDa (monomer)
~110 kDa (dimer)
Optimal pH: 7.5 to 9.0
Extinction Coefficient: E1% = 8.85 (PI) and 9.47 (PII) at 280 nm

Activators: Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds.

Inhibitors: sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate)

Unit Definition

One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C, unless otherwise indicated below.

Physical form

Lyophilized powder containing approx. 15% sodium citrate

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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