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H0413

Millipore

HIS-Select® Filter Plate

Sinónimos:

His-tag filtered microplate

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About This Item

UNSPSC Code:
41121800
NACRES:
NB.24

grade

for molecular biology

form

plate

storage temp.

2-8°C

General description

The HIS-Select® Filter Plate is an immobilized metal-ion affinity chromatography (IMAC) product that allows rapid purification of small-scale crude cell extracts containing histidine-tagged proteins. The HIS-Select® Filter Plate contains 20 μm, spherical silica particles (100 nm pore size) with a hydrophilic layer.

The HIS-Select® Filter Plate is selective for recombinant proteins with histidine tags and exhibits very low non-specific binding of other proteins. The selectivity can be modulated with the inclusion of imidazole during chromatography. The binding capacity of the HIS-Select® Filter Plate is typically > 2 mg per well as determined with an approximately 30 kDa histidine-tagged protein.

Application

The HIS-Select® Filter Plate is suitable for purification of recombinant proteins with histidine tags.

Features and Benefits

  • High Selectivity for his-tagged proteins
  • Ready-to-use plate format
  • Compatible with CelLytic™ cell lysis reagents

Legal Information

Covered by US Patent No 6,623,655 and are sold for research use only. Commercial use requires additional licenses.
CelLytic is a trademark of Sigma-Aldrich Co. LLC
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

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Referencia del producto
Descripción
Precios

pictograms

Health hazard
GHS08

signalword

Danger

Hazard Classifications

Aquatic Chronic 3 - Carc. 1A Inhalation - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1 - STOT RE 1 - STOT RE 2 Inhalation

target_organs

Respiratory Tract

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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An assay employing a panel of tumor-associated antigens has been validated and is available commercially (EarlyCDT®-Lung) to aid the early detection of lung cancer by measurement of serum autoantibodies. The high throughput (HTP) strategy described herein was pursued to identify
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The stability, activity, and solubility of a protein sequence are determined by a delicate balance of molecular interactions in a variety of conformational states. Even so, most computational protein design methods model sequences in the context of a single native

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