GE17-5268-02
Ni Sepharose™ High Performance
Cytiva 17-5268-02, pack of 100 mL
Sinónimos:
High Performance Ni Resin, Ni Sepharose
About This Item
Productos recomendados
packaging
pack of 100 mL
manufacturer/tradename
Cytiva 17-5268-02
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning in place
2-14(Ni2+-stripped medium.)
working range
3-12(Ni²⁺-stripped medium.)
capacity
≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)
Categorías relacionadas
General description
Ni Sepharose™ High Performance consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with Ni2+, which will selectively retain proteins with exposed histidine groups.
The high stability and broad compatibility of Ni Sepharose™ High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions.
Features and Benefits
- High-performance purification of histidine-tagged proteins
- Negligible leakage of Ni2+ ions.
- High binding capacity, at least 40 mg/mL medium
- Compatible with a wide range of reducing agents, detergents, denaturants and other additives
- 34 μm bead size for improved resolution
Storage and Stability
Analysis Note
Legal Information
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
This page shows a general procedure for cell lysis of recombinant histidine-tagged proteins using GE Healthcare products.
This page shows how to optimize purification of histidine-tagged proteins using GE Healthcare products.
This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using GE Healthcare products.
Protocolos
This page describes a small-scale purification (minipreps) of histidine-tagged recombinant proteins using His SpinTrap and His SpinTrap Kit from GE Healthcare.
This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose High Performance from GE Healthcare.
This page shows how to purify protein A-tagged proteins using GE Healthcare products.
This page how to isolate proteins from inclusion bodies using GE Healthcare products.
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