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Merck
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G9768

Sigma-Aldrich

Guanosine 5′-triphosphate–Agarose

aqueous glycerol suspension

Sinónimos:

5′-GTP agarose

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About This Item

MDL number:
MFCD00171655
UNSPSC Code:
41106500
NACRES:
NA.56

biological source

bacterial (CORYNEBACTERIUM SP)
enzyme from Porcine brain
plant (Sea weed)
yeast

Quality Level

200

form

aqueous glycerol suspension

extent of labeling

1-5 μmol per mL

matrix

cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

matrix attachment

ribose hydroxyls

matrix spacer

11 atoms (adipic acid dihydrazide)

storage temp.

−20°C

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Application

Guanosine 5′-triphosphate-agarose (5′-GTP agarose) is an agarose conjugate in aqueous glycerol suspension used in affinity chromatography, for purification of guanosine-binding proteis

Physical form

Guanosine 5′-triphosphate–Agarose has been used:
  • in chromatographic column for the purification of a translocation stimulating factor
  • in GTP-Agarose pull down assays to measure the levels of GTP-bound RHEB (Ras homolog enriched in brain)
  • in GTP-Binding assay
  • for the depletion of GTP binding proteins in cytosol of rat liver
Suspension in 50% glycerol containing 0.25 M NaCl

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

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P E Glaser et al.
Biochemistry, 34(38), 12193-12203 (1995-09-26)
Recently we demonstrated that the unique stereoelectronic relationships inherent in the structure of plasmenylethanolamine facilitate membrane fusion, and we postulated the existence of a membrane fusion protein which could exploit the propensity of plasmenylethanolamine molecular species to adapt an inverted
Arsenite stress down-regulates phosphorylation and 14-3-3 binding of leucine-rich repeat kinase 2 (LRRK2), promoting self-association and cellular redistribution
Mamais A, et al.
The Journal of Biological Chemistry, 289, 21386-21400 (2014)
Reiaz Ul Rehman et al.
Plant & cell physiology, 49(5), 751-766 (2008-04-04)
The regulatory functions of Rab proteins in membrane trafficking lie in their ability to perform as molecular switches that oscillate between a GTP- and a GDP-bound conformation. The role of tomato LeRab11a in secretion was analyzed in tobacco protoplasts. Green
M Bomsel et al.
The Journal of biological chemistry, 268(34), 25824-25835 (1993-12-05)
In endosomes the polymeric immunoglobulin receptor (pIgR) is sorted into transcytotic vesicles. Transcytosis of the pIgR in polarized Madin-Darby canine kidney (MDCK) cells is regulated both by phosphorylation of Ser664 and by binding of ligand, dimeric IgA (dIgA). Mutation of
Somatic mutations in TSC1 and TSC2 cause focal cortical dysplasia
American Journal of Human Genetics, 100(3), 454-472 (2017)
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