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G75120

Sigma-Aldrich

Sephadex® G-75

Medium

Sinónimos:

Gel filtration media, Gel filtration resin

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About This Item

Número de CAS:
37224-29-6
MDL number:
MFCD00132772
UNSPSC Code:
23151817
NACRES:
NA.56

Quality Level

200

technique(s)

buffer exchange: suitable

matrix active group

phase

swelling

1 g swells to 12-15 mL gel

bead diameter

40-120 μm

application(s)

life science and biopharma

compatibility

Cytiva

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General description

Sephadex G-75 is widely used for desalting and buffer exchange. This gel filtration resin is created through the crosslinking of dextran with epichlorohydrin. Sephadex varies in its cross-linking level across different types, influencing its swelling capacity and molecular fractionation range.
Sephadex® G-75 is a gel filtration media used in gel filtration chromatography and protein chromatography. Sephadex® G-75 has been used to study cystatin of the mammalian brain, suggest candidates for development of novel oral or other anti-infective agents and to purify procoagulant metalloproteinase from rattlesnake venom.

Application

Sephadex® G-75 has been used to purify low density lipoproteins (LDL) by gel filtration.
Sephadex® G-75 has been used to separate the products from the pepsin digest by gel filtration chromatography and to desalt low density lipoproteins (LDL) by gel filtration.

Features and Benefits

  • Enables rapid desalting, contaminant removal, and buffer exchange in a single step.
  • Ideal for buffer exchange and cleanup of large biomolecules with a molecular weight exceeding 80,000.
  • Features the highest exclusion limit of 80,000 within the Sephadex G-range.
  • Classic gel filtration resin.

Legal Information

Sephadex is a registered trademark of Cytiva

replaced by

Referencia del producto
Descripción
Precios

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Inhibition of cyclic AMP-and cyclic GMP-mediated dilations in isolated arteries by oxidized low density lipoproteins.
Galle J, et al.
Arteriosclerosis and Thrombosis, 12(2), 180-186 (1992)
Oxidized low density lipoproteins potentiate vasoconstrictions to various agonists by direct interaction with vascular smooth muscle.
Galle J, et al.
Circulation Research, 66(5), 1287-1293 (1990)
Jose Munoz-Munoz et al.
mBio, 12(4), e0136821-e0136821 (2021-08-04)
The human gut microbiota (HGM) contributes to the physiology and health of its host. The health benefits provided by dietary manipulation of the HGM require knowledge of how glycans, the major nutrients available to this ecosystem, are metabolized. Arabinogalactan proteins
Rapid purification of fluorescent dye-labeled products in a 96-well format for high-throughput automated DNA sequencing.
K Krakowski et al.
Nucleic acids research, 23(23), 4930-4931 (1995-12-11)
Fakhra Amin et al.
Protein and peptide letters, 18(2), 210-218 (2010-11-09)
Cystatins are thiol proteinase inhibitors ubiquitously present in mammalian body and serve various important physiological functions. To purify and characterize Thiol protease inhibitor from buffalo brain and to compare its properties with respect to tissue and organ difference from other
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