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G1166

Sigma-Aldrich

Monoclonal Anti-Glutamic Acid Decarboxylase 65 antibody produced in mouse

clone GAD-6, purified immunoglobulin, buffered aqueous solution

Sinónimos:

Anti-GAD 65

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About This Item

MDL number:
MFCD03455043
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

GAD-6, monoclonal

form

buffered aqueous solution

species reactivity

mammals (and some lower vertebrates)

technique(s)

immunohistochemistry: 1:100-1:500
western blot: 1:1,000-1:5,000

isotype

IgG2a

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GAD2(2572)
mouse ... Gad2(14417)

Specificity

Recognizes the lower molecular weight isoform of the two GAD isoforms identified in brain, GAD 65. Can be used for immunochemical localization of GABA secreting neurons.

Immunogen

purified rat brain GAD 65.

Physical form

Solution in 0.01 M phosphate buffered saline, containing 0.04% sodium azide.

Preparation Note

Purified using Protein A chromatography.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Gregory J Brewer et al.
Frontiers in neural circuits, 7, 165-165 (2013-10-25)
The mammalian hippocampus functions to encode and retrieve memories by transiently changing synaptic strengths, yet encoding in individual subregions for transmission between regions remains poorly understood. Toward the goal of better understanding the coding in the trisynaptic pathway from the
M Esclapez et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 14(3 Pt 2), 1834-1855 (1994-03-01)
Two isoforms of glutamic acid decarboxylase (GAD67 and GAD65) and their mRNAs were localized in the rat brain by immunohistochemistry and nonradioactive in situ hybridization methods with digoxigenin-labeled cRNA probes. In most brain regions, both GAD isoforms were present in
Seung T Lim et al.
The Journal of comparative neurology, 507(2), 1228-1244 (2008-01-09)
Nectins are cell adhesion molecules that, together with the intracellular binding partner afadin, mediate adhesion and signaling at a variety of intercellular junctions. In this work we studied the distribution of nectin-1 and afadin during hippocampal synapse formation using cultured
Ran-Sook Woo et al.
Neuron, 54(4), 599-610 (2007-05-25)
Neuregulin-1 (NRG1), a regulator of neural development, has been shown to regulate neurotransmission at excitatory synapses. Although ErbB4, a key NRG1 receptor, is expressed in glutamic acid decarboxylase (GAD)-positive neurons, little is known about its role in GABAergic transmission. We
Stathis S Leondopulos et al.
Journal of neural engineering, 9(2), 026015-026015 (2012-03-01)
Slow wave oscillations in the brain are essential for coordinated network activity but have not been shown to self-organize in vitro. Here, the development of dissociated hippocampal neurons into an active network with oscillations on multi-electrode arrays was evaluated in
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