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Merck

F5137

Sigma-Aldrich

Anti-Sheep IgG (whole molecule)−FITC antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Sinónimos:

Rabbit Anti-Sheep IgG (whole molecule)−Fluorescein isothiocyanate

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

conjugate

FITC conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

storage condition

protect from light

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
indirect immunofluorescence: 1:100

storage temp.

−20°C

target post-translational modification

unmodified

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Anti-Sheep IgG (whole molecule)-FITC antibody is specific for sheep IgG. Rabbit Anti-Sheep IgG is conjugated to Fluorescein Isothiocyanate (FITC) in an alkaline reaction, then further purified to remove unbound FITC.

Immunogen

Purified sheep IgG

Application

Anti-Sheep IgG (whole molecule) FITC antibody produced in rabbit has been used in indirect immunofluorescence assay (IFAT) and immunofluorescence staining.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Yi Wen et al.
American journal of physiology. Renal physiology, 318(1), F107-F116 (2019-11-19)
Nephrotoxic serum nephritis (NTN) models immune-mediated human glomerulonephritis and culminates in kidney inflammation and fibrosis, a process regulated by T lymphocytes. TNF-α is a key proinflammatory cytokine that contributes to diverse forms of renal injury. Therefore, we posited that TNF-α
Larissa Martins de Brito Moraes et al.
Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria, 20(4), 312-317 (2011-12-15)
Neosporosis and toxoplasmosis are parasitic diseases which can cause reproductive problems in goats and sheep. The current study aimed to determine the occurrence of anti-Neospora caninum and anti-Toxoplasma gondii IgG antibodies in goats and sheep from the districts of Amarante
Ramon G B Bonegio et al.
Journal of the American Society of Nephrology : JASN, 16(7), 2063-2072 (2005-05-27)
Proteinuria is a risk factor for progression of chronic renal failure. A model of proteinuria-associated tubulointerstitial injury was developed and was used to examine the therapeutic effect of rapamycin. Two studies were performed. In study A, proteinuric rats were given
Occurrences of anti-Toxoplasma gondii and anti-Neospora caninum antibodies in sheep from four districts of Tocantins state, Brazilian Legal Amazon Region
Guimaraes A, et al.
Pesquisa Veterinária Brasileira, 35(2), 110-114 (2015)
Janine A Duckworth et al.
Reproduction (Cambridge, England), 133(1), 177-186 (2007-01-25)
In a previous study, three infertility-relevant epitopes of possum ZP2 (Pep12 (amino acids 111-125), Pep31 (amino acids 301-315), and Pep44 (amino acids 431-445)) were identified using sera from possums (Trichosurus vulpecula) immunized with recombinant possum zona pellucida 2 (ZP2) constructs

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