DUO92102
Duolink® In Situ Orange Starter Kit Mouse/Rabbit
Sinónimos:
in situ Proximity Ligation Assay reagent, Protein Protein Interaction Assay reagent
About This Item
Productos recomendados
product line
Duolink®
technique(s)
proximity ligation assay: suitable
fluorescence
λex 554 nm; λem 576 nm (orange) (Cyanine 3; Zeiss Filter set 20)
suitability
suitable for fluorescence
storage temp.
−20°C
Categorías relacionadas
Application
Follow the Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.
Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.
To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes.This starter kit supplies all other necessary reagents for 30 Duolink® PLA reactions, which include a pair of PLA probes (Anti-Rabbit PLUS and Anti-Mouse MINUS), orange detection reagents, wash buffers, and mounting medium.Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
The Duolink® In Situ Orange Starter Kit Mouse/Rabbit requires one primary antibody from mouse and one primary antibody from rabbit. Orange fluorescence detection reagents are often used with Cyanine 3 filter.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
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Features and Benefits
- No overexpression or genetic manipulation required
- High specificity (fewer false positives)
- Single molecule sensitivity due to rolling circle amplification
- Relative quantification possible
- No special equipment needed
- Quicker and simpler than FRET
- Increased accuracy compared to co-IP
- Publication-ready results
Legal Information
Los componentes del kit también están disponibles por separado
- DUO92002Duolink® In Situ PLA® Probe Anti-Rabbit PLUS, Affinity purified Donkey anti-Rabbit IgG (H+L)SDS
- DUO92004Duolink® In Situ PLA® Probe Anti-Mouse MINUS, Affinity purified Donkey anti-Mouse IgG (H+L)SDS
- DUO92007Duolink® In Situ Detection Reagents OrangeSDS
- DUO82049Duolink® In Situ Wash Buffers, FluorescenceSDS
- DUO82040Duolink® In Situ Mounting Medium with DAPISDS
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Sens. 1
Storage Class
8A - Combustible corrosive hazardous materials
wgk_germany
WGK 3
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Artículos
Learn how Proximity Ligation Assay technology works and how the protein-protein interaction control kit can confirm in situ detection of EGF-induced EGFR-HER2 dimerization.
Duolink proximity ligation assay used to study neuron interactions furthering neuroscience research.
Find Duolink references based on the type of method used, post translational modification detected, and research focus.
Contenido relacionado
Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.
Applications to detect, quantify and visualize protein-protein interactions, post-translational modifications and low expression protein detection using proximity ligation assay
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