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D8144

Sigma-Aldrich

Dextranase from Penicillium sp.

lyophilized powder, 400-800 units/mg protein

Sinónimos:

1,6-α-D-Glucan 6-glucanohydrolase

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About This Item

Número de CAS:
9025-70-1
Comisión internacional de enzimas:
3.2.1.11 (BRENDA, IUBMB)
EC Number:
232-803-9
MDL number:
MFCD00130940
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Penicillium sp.

Quality Level

200

form

lyophilized powder

specific activity

400-800 units/mg protein

composition

Protein, 35% Lowry

storage temp.

2-8°C

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General description

Dextranase from fungi belong to the glycoside hydrolase families (GH) 49. Dextranase from Penicillium sp. are stable at varying temperature and pH.

Application

Dextranase from Penicillium sp has been used:
  • in the digestion of exopolysaccharides from P. aeruginosa biofilms and dextran polymers
  • as a component of trypticase soy broth (TSYPS) for Streptococcus mutans culture
  • in microgel degradation

Biochem/physiol Actions

An endodextranase that hydrolyzes -(1,6)-glucosidic linkages in dextran. Dextrans are undesirable compounds synthesized from sucrose by microbial contaminants during sugar production that increase viscosity of the flow and decrease industrial recovery. Dextranase has been used for hydrolyzing dextran at sugar mills in order to improve efficiency of sugar production.

Unit Definition

One unit will liberate 1.0 μmole of isomaltose (measured as maltose) per min at pH 6.0 at 37 °C, using dextran as substrate.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

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Label-free high-throughput functional lytic assays
O'Malley SM, et al.
Journal of Biomolecular Screening, 12(1), 117-125 (2007)
Biology and genome sequence of Streptococcus mutans phage M102AD
Delisle AL, et al.
Applied and Environmental Microbiology, 78(7), 2264-2271 (2012)
T I Danilova et al.
Prikladnaia biokhimiia i mikrobiologiia, 19(1), 104-110 (1983-01-01)
The carbohydrate composition of dextranase from Penicillium funiculosum 15, as well as the composition of products of dextran deep hydrolysis by the enzyme were studied. The products are normally used to stabilize the enzyme during its purification. Using the methods
Dextranase from Penicillium minioluteum: reaction course, crystal structure, and product complex
Larsson AM, et al.
Structure, 11(9), 1111-1121 (2003)
Hordenine: a novel quorum sensing inhibitor and antibiofilm agent against Pseudomonas aeruginosa
Zhou JW, et al.
Journal of Agricultural and Food Chemistry, 66(7), 1620-1628 (2018)
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