FGC4

94101904

• UNSPSC Code: 41106514

Propiedades

• biological source: rat skin
• growth mode: Adherent
• karyotype: Modal no. 52
• morphology: Epithelial
• products: Albumin and ß-fibrogen
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Rat hepatoma [alb-gpt plasmid]

Cell Line Description

A subclone derived from Fao which is deficient in HGPRT (resistant to 5μg/ml 6-thioguanine and also resistant to 3mM ouabain). FGC4 contains the alb-gpt plasmid which is the bacterial gpt gene under control of the rat albumin promoter (400 bp). Produces albumin and ß-fibrinogen.GMO1

Application

Gene expression studies.

Culture Medium

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Subculture Routine

Split semi-confluent cultures (70%) 1:100 i.e. seeding at 2x1000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Media change three times a week.

Other Notes

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