BXPC3 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93120816, human pancreas, Epithelial

• Sinónimos: Biopsy Xenograft of Pancreatic Carcinoma Cell Line-3, BxPC-3 Cells
• UNSPSC Code: 41106514

Propiedades

• biological source: human pancreas
• description: Human primary pancreatic adenocarcinoma
• packaging: tube of 5 μg 93120816-DNA-5UG; pkg of vial of cells 93120816-1VL
• growth mode: Adherent
• karyotype: 2n = 59, near triploid
• morphology: Epithelial
• products: Mucin
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Human primary pancreatic adenocarcinoma

Cell Line Description

Derived from a 61 year old female with a primary adenocarcinoma of the pancreas. BxPC-3 cells produce mucin and the tumour produced in a nude mouse is moderately well to poorly differentiated like the primary adenocarcinoma.

Application

BXPC3 cell line has been used to determine the biological and therapeutic importance of gastrin gene expression in pancreatic adenocarcinomas.

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 13
D13S317: 11
D16S539: 9,11
D5S818: 11
D7S820: 10,13
THO1: 9
TPOX: 8
vWA: 14,18

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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