NFS-25 C-3

93090713, mouse blood, Lymphoblast

• UNSPSC Code: 41106514

Propiedades

• Nombre del producto: NFS-25 C-3, 93090713
• biological source: mouse blood
• packaging: tube of 5 μg 93090713-DNA-5UG; pkg of vial of cells 93090713-1VL
• growth mode: Suspension
• karyotype: Not specified
• morphology: Lymphoblast
• products: Not specified
• receptors: Ly-17, Lyb-2, Ly-1, Ly-5 (B220)
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse pre-B lymphoblast

Cell Line Description

Derived from a spontaneous lymphoma in an NFS.C58v-1 mouse. These pre-B cells are negative for surface Ig, Ia and ThB.

Culture Medium

DMEM + 10% NCTC 109 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 1mM Oxaloacetic acid + 50μM 2-Mercaptoethanol (2ME) + 2μg/ml Transferrin + 0.2IU/ml Insulin + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Immediately after resuscitation a pre-centrifugation step to remove cryoprotectant (DMSO) is recommended i.e. pellet the cells by centrifugation at 100 x g for 5 minutes and resuspend the cell

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.