RO82-W-1

92030502

• UNSPSC Code: 41106514

Propiedades

• biological source: human thyroid
• growth mode: Adherent
• karyotype: 2n = 60-82, 82 predominant
• morphology: Epithelial
• products: Thyroglobulin
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): metastasis
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Human follicular thryoid carcinoma

Cell Line Description

Established from the metastases of a follicular carcinoma in a female patient. Tumorigenic in nude mice. The basal release of immuno-detectable thyroglobulin increased in response to thyroid-stimulating hormone (TSH). The cell line grows as a monolayer without the formation of follicles. Electronmicroscopy studies showed the presence of microvilli with interdigitations between neighbouring cells.

Application

A valuable model for the study of human follicular carcinoma.

Culture Medium

DMEM:Ham′s F12:MCDB 105 (2:1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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