70Z/3

91092002, mouse blood, Lymphoblastoid

• UNSPSC Code: 41106514

Propiedades

• product name: 70Z/3, 91092002
• biological source: mouse blood
• growth mode: Suspension
• karyotype: Not specified
• morphology: Lymphoblastoid
• products: Cytoplasmic Immunoglobulin
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse pre-B lymphocyte

Cell Line Description

Derived from a methyl nitrosourea-induced tumour in a (C57BL/6XDBA/2) F1 mouse. This line is positive for cytoplasmic Ig and negative for surface membrane Ig and TdT.

Culture Medium

RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 2-5x100,000 cells/ml; 5% CO₂; 37°C.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Dr P Kincade, Oklahoma Medical Research Foundation