SW 948

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 91030714, human colon, Epithelial

• UNSPSC Code: 41106514

Propiedades

• biological source: human colon
• packaging: tube of 5 μg 91030714-DNA-5UG; pkg of vial of cells 91030714-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Carcinoembryonic antigen (CEA)
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Human Caucasian colon adenocarcinoma

Cell Line Description

Derived from a grade III adenocarcinoma of the colon of an 81 year old Caucasian female. The cells synthesise relatively large amounts of CEA (2,000ng/1,000,000 cells in 21 days).

Application

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 10,11
D16S539: 11,12
D5S818: 11
D7S820: 9,11
THO1: 6,9.3
TPOX: 8,11
vWA: 16,18

Culture Medium

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; No CO₂; 37°C.

Other Notes

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