Detroit 562

87042205, human pharynx, Heteroploid

• UNSPSC Code: 41106514

Propiedades

• product name: Detroit 562, 87042205
• biological source: human pharynx
• growth mode: Adherent
• karyotype: 2n = 46
• morphology: Heteroploid
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Human Caucasian pharynx carcinoma

Cell Line Description

Derived from pleural fluid of an adult female with primary carcinoma of the pharynx. The cells have the type B glucose-6-phosphate dehydrogenase.

Application

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11,13
D13S317: 12
D16S539: 11
D5S818: 11,12
D7S820: 8,10
THO1: 8,9
TPOX: 8,10
vWA: 16

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 0.1% Lactalbumin hydrolysate (LH) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Other Notes

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