OX-114

13082227, from Rat x Rat Hybridoma

• UNSPSC Code: 41106514

Propiedades

• biological source: Rat x Rat Hybridoma
• ImmunoDon: Rat DA spleen
• Myeloma: Y3
• Reactivity: Mouse CD147
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Description

The hybridoma cell line OX-114 recognises mouse CD147 secretes monoclonal IgG1 antibodies reactive with rat L-selectin.

Immunogen

Recombinant CD147

Application

The isotype of the monoclonal antibody produced by this cell line is rat IgG1.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

Subculture Routine

Seed at 5 x 10⁴ cells/ml (5 x 10⁵ cells/ml at resuscitation). Subculture when cells reach 5 x 10⁵ - 1 x 10⁶ cells/ml (usually after 3-4 days), 5% CO₂, 37°C. 20% FBS can be used at resuscitation.

Other Notes

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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.