OX-80

from Rat x Rat Hybridoma, 13082211

• UNSPSC Code: 41106514

Propiedades

• biological source: Rat x Rat Hybridoma
• ImmunoDon: BALB/c mouse spleen
• Myeloma: Y3
• Reactivity: Mouse CD53
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Description

The hybridoma cell line OX-80 secretes monoclonal IgM antibodies reactive with CD53.

Immunogen

RAW 264 cells

Application

The isotype of the monoclonal antibody produced by this cell line is rat IgM.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

Subculture Routine

Seed at 5 x 10⁴ cells/ml (5 x 10⁵ cells/ml at resuscitation). Subculture when cells reach 5 x 10⁵ - 1 x 10⁶ cells/ml (usually after 3-4 days), 5% CO₂, 37°C. 20% FBS can be used at resuscitation.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.