AB-1 HA

12070506, mouse mesothelium, epithelial; small, spindly morphology, cobblestone at confluence

• Sinónimos: AB 1 HA, AB-1HA
• UNSPSC Code: 41106514

Propiedades

• Nombre del producto: AB-1 HA, 12070506
• biological source: mouse mesothelium
• packaging: tube of 5 μg 12070506-DNA-5UG; pkg of vial of cells 12070506-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial; cells are small and can be spindly at low density but form a uniform cobblestone epithelial layer at near confluence
• receptors: Not specified
• relevant disease(s): cancer

Descripción

Cell Line Origin

Mouse mesothelioma cell line transfected with influenza haemagglutinin gene (HA)

Cell Line Description

AB-1 HA is a mouse mesothelioma cell line transfected with influenza haemagglutinin gene (HA). Mice (BALB/c strain, female, 6-8 weeks old) were exposed to crocidolite asbestos through intraperitoneal injection, resulting in tumour development. Cultures were established from malignant mesothelial cells obtained from ascites fluid. The resulting celll ine, designated AB-1 was transfected with influenza haemagglutinin gene (HA) in order to generate an endogenous tumour antigen. Cell Bank Australia reference number: CBA1374.

DNA Profile

Not specified

Culture Medium

RPMI 1640 (R0883) (with 2 mM L-Glutamine (G7513) + 25mM HEPES) + FBS / FCS (F2442) + 400ug/ml G418.

Subculture Routine

Optimal split ratio 1:10 - 1:20 (seeding density 0.7 x 10⁴ cells/cm²). Cells incubated at 37 °C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min. Cells will require passaging twice weekly at this seeding density.

Other Notes

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Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Richard Lake - University of Western Australia, Australia