MM485

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 11072803, human skin, Epithelial

• Sinónimos: MM 485
• UNSPSC Code: 41106514

Propiedades

• biological source: human skin
• packaging: tube of 5 μg 11072803-DNA-5UG; pkg of vial of cells 11072803-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): metastasis
• shipped in: dry ice

Descripción

Cell Line Origin

Human melanoma metastatic site lymph node

Cell Line Description

MM485 is a human melanoma derived from a metastatic site (lymph node). Cell Bank Australia reference number CBA-1355

Culture Medium

RPMI 1640 (R0883) (with 2 mM L-L-Glutamine (G7513) + 25 mM HEPES (H0887) + 10% FBS / FCS (F2442).

Subculture Routine

Optimal split ratio 1:4 (seeding density 1.2 x 10⁴ cells/cm²). Cells incubated at 37 °C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments.  Please check with your local Customer Service representative for more information.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.