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C4018

Sigma-Aldrich

Choloylglycine Hydrolase from Clostridium perfringens (C. welchii)

lyophilized powder, ≥100 units/mg protein

Sinónimos:

3α,7α,12α-Trihydroxy-5β-cholan-24-oylglycine amidohydrolase

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About This Item

Número de CAS:
37289-07-9
Comisión internacional de enzimas:
3.5.1.24 (BRENDA, IUBMB)
MDL number:
MFCD00130789
UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized powder

specific activity

≥100 units/mg protein

composition

Protein, ≥50% biuret

shipped in

wet ice

storage temp.

−20°C

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Application

The enzyme from Sigma has been used in the analysis of bile samples in various studies.

Biochem/physiol Actions

The enzyme is an N-terminal nucleophilic (Ntn) hydrolase that catalyzes the hydrolysis of amide bonds, liberates the glycine/taurine moiety from the steroid core and eventually yields unconjugated bile acids. Agents that oxidize thiol groups (e.g., p-mercuribenzoate, iodoacetamide, Hg2+, Cu2+, and Cd2+) have been shown to strongly inhibit bile salt hydrolase (BSH) activity in Clostridium perfringens.

Unit Definition

One unit will hydrolyze 1.0 μmole of glycocholic acid to glycine and cholic acid in 5 min at pH 5.6 at 37 °C.

Physical form

Partially purified lyophilized powder containing buffer salts and stabilizer

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Bile salt hydrolase activity in probiotics.
Máire Begley et al.
Applied and environmental microbiology, 72(3), 1729-1738 (2006-03-07)
Effects of Quercetin and Rutin on Serum and Hepatic Lipid Concentrations, Fecal Steroid Excretion and Serum Antioxidant Properties
Nakamura Y, et al.
Journal of Health Science, 46(4), 229-240 (2000)
Maksim Rossocha et al.
Biochemistry, 44(15), 5739-5748 (2005-04-13)
Bacterial bile salt hydrolases catalyze the degradation of conjugated bile acids in the mammalian gut. The crystal structures of conjugated bile acid hydrolase (CBAH) from Clostridium perfringens as apoenzyme and in complex with taurodeoxycholate that was hydrolyzed to the reaction
Anil K Patel et al.
Applied biochemistry and biotechnology, 162(1), 166-180 (2009-08-12)
Probiotic has modernized the current dietetic sense with novel therapeutic and nutritional benefits to the consumers. The presence of bile salt hydrolase (BSH) in probiotics renders them more tolerant to bile salts, which also helps to reduce the blood cholesterol
F Kannenberg et al.
The Journal of biological chemistry, 274(50), 35455-35460 (1999-12-10)
Peroxisomal beta-oxidation plays an important role in the metabolism of a wide range of substrates, including various fatty acids and the steroid side chain in bile acid synthesis. Two distinct thiolases have been implicated to function in peroxisomal beta-oxidation: the
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Protocolos

Enzymatic Assay of Choloylglycine Hydrolase

To standardize a procedure for the determination of the enzymatic assay of choloylglycine hydrolase.

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