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C1154

4-Chloro-7-sulfobenzofurazan ammonium salt

Name: 4-Chloro-7-sulfobenzofurazan ammonium salt
Brand: SIGMA

Powder

Sinónimos:

4-Chloro-7-sulfobenz-2-oxa-1,3-diazole ammonium salt, 7-Chloro-2,1,3-benzoxadiazole-4-sulfonic acid ammonium salt, 7-Chloro-4-benzofurazansulfonic acid ammonium salt, SBF-Chloride

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About This Item

Fórmula lineal:

C₆H₃ClN₂O₄S · NH₃

Número de CAS:

81377-14-2

Peso molecular:

251.65

Beilstein/REAXYS Number:

5200152

MDL number:

MFCD00038995

UNSPSC Code:

12171500

PubChem Substance ID:

24892366

NACRES:

NA.47

product name

4-Chloro-7-sulfobenzofurazan ammonium salt,

assay

>98%

form

powder

color

brown
light yellow

mp

>300 °C (lit.)

solubility

water: 50 mg/mL, clear, dark yellow

fluorescence

λ_ex 360 nm; λ_em 410 nm (pH 8.5)
λ_ex 383 nm; λ_em 528 nm (Reaction product)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

N.OS(=O)(=O)c1ccc(Cl)c2nonc12

InChI

1S/C6H3ClN2O4S.H3N/c7-3-1-2-4(14(10,11)12)6-5(3)8-13-9-6;/h1-2H,(H,10,11,12);1H3

InChI key

QFHXPBLOMHGYOC-UHFFFAOYSA-N

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pictograms

GHS07

signalword

Warning

hcodes

H315,H319,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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The nature of the reaction of an essential tyrosine residue of bovine heart mitochondrial ATPase with 4-chloro-7-nitrobenzofurazan and related compounds.

R Sutton et al.

European journal of biochemistry, 142(2), 387-392 (1984-07-16)

Bovine heart mitochondrial ATPase is inhibited after covalent modification with 4-chloro-7-nitrobenzofuroxan. The kinetics of the reaction are indistinguishable from those for the reaction of an essential tyrosine residue of the ATPase with 4-chloro-7-nitrobenzofurazan that have been described previously [Ferguson et

Chromatographic separation of fluorescent thiol adducts of 4-chloro-7-sulphobenzofurazan. Use as substrates for enzymes of the mercapturic acid xenobiotic pathway.

X P Chen et al.

Journal of chromatography. B, Biomedical sciences and applications, 709(1), 19-25 (1998-07-08)

Fluorescent adducts of 4-chloro-7-sulphobenzofurazan with cysteine, cysteinylglycine, reduced glutathione and N-acetylcysteine were prepared. Adducts were separated by HPLC on a 3-mm Nova-Pak C18 reversed-phase column using isocratic elution with a solvent of acetonitrile-0.15 M phosphoric acid (5:95) buffered at pH

Ammonium 4-chloro-7-sulfobenzofurazan: a new fluorigenic thiol-specific reagent.

J L Andrews et al.

Archives of biochemistry and biophysics, 214(1), 386-396 (1982-03-01)

Ammonium 4-chloro-7-sulfobenzofurazan: a fluorescent substrate highly specific for rat glutathione S-transferase subunit 3.

R M Bolton et al.

Analytical biochemistry, 216(2), 418-423 (1994-02-01)

Ammonium 4-chloro-7-sulfobenzofurazan (Sbf-Cl) is a water-soluble fluorescent reagent which is highly specific for thiol groups. We describe here a simple and sensitive fluorescence assay which is highly specific for subunit 3 of the rat glutathione S-transferases. Specific activities of isoenzymes

Quercetin Increases Hepatic Homocysteine Remethylation and Transsulfuration in Rats Fed a Methionine-Enriched Diet.

Bin Meng et al.

BioMed research international, 2015, 815210-815210 (2015-11-12)

This study was aimed at investigating the effects of quercetin on mRNA expression and activity of critical enzymes in homocysteine metabolism in rats fed a methionine-enriched diet. Rats were fed for 6 weeks the following diets, that is, control, 0.5%

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