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A8678

Sigma-Aldrich

Agar

suitable for plant cell culture

Sinónimos:

Agar goma, Agar-agar

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About This Item

Fórmula lineal:
(C12H18O9)n
Número de CAS:
9002-18-0
EC Number:
232-658-1
MDL number:
MFCD00081288
UNSPSC Code:
50405901
NACRES:
NA.21

biological source

algae (Rhodophyceae)

Quality Level

200

form

powder

technique(s)

cell culture | plant: suitable

mp

80-90 °C (1.5% in water)

transition temp

transition temp 27-37 °C (1.5%)

application(s)

agriculture

storage temp.

room temp

InChI

1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1

InChI key

GYYDPBCUIJTIBM-DYOGSRDZSA-N

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General description

Agar or Agar-agar is one of the major components found in the cell wall of red algae. It is a polysaccharide complex, comprising about 70% agarose and 30% agaropectin.

Application

Washed agar has been used:
  • as a supplement (0.9% w/v) to Murashige and Skoog basal medium for plant growth
  • in the preparation of cocultivation medium for Agrobacterium tumefaciens mediated transformation in maize (Zea mays) hybrid Hi-II
  • as a component in the complete medium to regenerate protoplasts of Piriformospora indica
  • as a clean agar medium to culture Synechococcus WH7803 strain

Biochem/physiol Actions

Agar is an ester of sulfuric acid in a linear galactan form, that makes it soluble in hot water and insoluble in cold. Washed agar contain less impurities and low level of nutrients.

Preparation Note

Washed

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

ppe

Eyeshields, Gloves, type N95 (US)

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Alga Zuccaro et al.
Fungal genetics and biology : FG & B, 46(8), 543-550 (2009-04-09)
Piriformospora indica (Basidiomycota, Sebacinales) is a root colonizing fungus which is able to increase biomass and yield of crop plants and to induce local and systemic resistance to fungal diseases and tolerance to abiotic stress. A prerequisite for the elucidation
Andrew D Millard
Methods in molecular biology (Clifton, N.J.), 501, 33-42 (2008-12-11)
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Arnaud Dechesne et al.
Applied and environmental microbiology, 74(16), 5195-5200 (2008-07-01)
Water is arguably the most important constituent of microbial microhabitats due to its control of physical and physiological processes critical to microbial activity. In natural environments, bacteria often live on unsaturated surfaces, in thin (micrometric) liquid films. Nevertheless, no experimental
Queeny Wing-Han Yuen et al.
The open biomedical engineering journal, 5, 39-46 (2011-06-07)
Cervical lymph nodes are common sites of metastatic involvement in head and neck cancers. These lymph nodes are superficially located and palpation is a common practice for assessing nodal hardness and staging cancer which is, however, too subjective and with
GunNam Na et al.
The Journal of biological chemistry, 286(47), 40423-40432 (2011-09-21)
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