Saltar al contenido
Merck

A0295

Sigma-Aldrich

Anti-Human IgA (α-chain specific)−Peroxidase antibody produced in goat

affinity isolated antibody

Sinónimos:

Goat Anti-Human IgA (α-chain specific)−HRP

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

concentration

4-11 mg/mL

technique(s)

direct ELISA: 1:50,000
dot blot: 1:80,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
western blot: suitable

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

¿Está buscando productos similares? Visita Guía de comparación de productos

General description

Anti-Human IgA (a-chain specific)-peroxidase antibody is isolated from goat anti-human IgA antiserum by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the alpha chain of human IgA.Goat antihuman IgA is conjugated to peroxidase and purified to remove unconjugated material. Specificity of the peroxidase conjugated anti-human IgA is determined by immunoelectrophoresis (IEP) and cross-reactivity of the conjugate is determined by enzyme linked immunoassays (ELISA).

Specificity

Anti-Human IgA (a-chain specific)-Peroxidase antibody produced in goat conjugate is specific for human IgA when tested against human IgA, IgG, IgM, Bence Jones Kappa and Lambda myeloma proteins. The antibody conjugate does not react with mouse or rat IgG.

Immunogen

Purified human IgA.

Application

Anti-Human IgA (α-chain specific) Peroxidase antibody produced in goat has been used in:
  • Enzyme linked immunosorbent assays (ELISA) at dilution of 1:3,000 and 1: 1,600
  • Tissue anti-transglutaminase antibody (tTG) assay at a dilution of 1:1,000
  • Dot blot: a minimum working dilution of 1:80,000 was determined in a direct chemiluminescence assay using 10ng human IgA/dot. Luminol plus enhancer was used as substrate.
  • Immunohistology using formalin-fixed, paraffin-embedded sections at a dilution of 1:100
  • Transcytosis assay of dimeric IgA (D-IgA)
  • Western Blotting

Biochem/physiol Actions

IgA is a secretory glycoprotein antibody that regulates the physiological balance between commensal intestinal bacteria and the defenses of the host immune system. It is the highest generated antibody isotype. IgAs are mainly secreted in the intestinal mucosa and prevent the commensal microorganisms from invading the intestines.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT as preservative

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Storage and Stability

For continuous use, store at 2-8°C for a maximum of one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

¿No encuentra el producto adecuado?  

Pruebe nuestro Herramienta de selección de productos.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Allergic Colitis in Infants Related to Cow
Yu MC, et al.
Pediatrics, 54(1), 49-55 (2013)
Age Dependence and Isotype Specificity of Influenza Virus Hemagglutinin Stalk-Reactive Antibodies in Humans
Nachbagauer R, et al.
mBio, 7(1), 01996-01915 (2016)
An unusual cause of postoperative dyspnoea.
B Cadet et al.
Gut, 53(9), 1278-1278 (2004-08-13)
Ole J B Landsverk et al.
The Journal of experimental medicine, 214(2), 309-317 (2017-01-21)
Plasma cells (PCs) produce antibodies that mediate immunity after infection or vaccination. In contrast to PCs in the bone marrow, PCs in the gut have been considered short lived. In this study, we studied PC dynamics in the human small
Prevalence of celiac disease among blood donors in SAO PAULO - the most populated city in Brazil
Alencar ML, et al.
Clinics, 67(9), 1013-1013 (2012)

Artículos

Antibody-based serology tests are useful in identifying subjects with an adaptive immune response to the SARS-CoV-2 virus. Anti-human immunoglobulin antibodies allow for quick and simple, yet reliable assays with easy readouts and can also be adapted for high-throughput screening.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico