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Merck

A0156

Sigma-Aldrich

N-(4-Aminobutyl)-N-ethylisoluminol

≥90%, powder

Sinónimos:

4-(N1-Ethyl-4-aminobutylamino)phthalic hydrazide, 6-[N-(4-Aminobutyl)-N-ethylamino]-2,3-dihydro-1,4-phthalazinedione, ABEI

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About This Item

Fórmula empírica (notación de Hill):
C14H20N4O2
Número de CAS:
Peso molecular:
276.33
Beilstein/REAXYS Number:
920895
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83

Nombre del producto

N-(4-Aminobutyl)-N-ethylisoluminol, ≥90%

Quality Level

assay

≥90%

form

powder

mp

259-260 °C (lit.)

solubility

glacial acetic acid: 50 mg/mL, clear, colorless to yellow

storage temp.

2-8°C

SMILES string

CCN(CCCCN)c1ccc2C(=O)NNC(=O)c2c1

InChI

1S/C14H20N4O2/c1-2-18(8-4-3-7-15)10-5-6-11-12(9-10)14(20)17-16-13(11)19/h5-6,9H,2-4,7-8,15H2,1H3,(H,16,19)(H,17,20)

InChI key

LEOJISUPFSWNMA-UHFFFAOYSA-N

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Features and Benefits

Efficient chemiluminescent NH2-coupling reagent for detection of a wide variety of proteins down to the picomole range.

Other Notes

Reportedly, the use of this material in chemiluminescent assays has distinct advantages over conventional radioimmunoassay.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Shulin Zhao et al.
Chemical communications (Cambridge, England), 48(5), 699-701 (2011-12-03)
We report on a novel strategy to improve microfluidic immunoassay sensitivity by introducing chemiluminescence resonance energy transfer (CRET) into the immunoreactions. The proposed CRET-based immunoassay for estradiol (E2, as a model analyte) is one of the most sensitive immunoassay with
L Farina et al.
Journal of bioluminescence and chemiluminescence, 4(1), 587-593 (1989-07-01)
We have developed a two-site immunoassay for human prolactin using two monoclonal antibodies: one of them immobilized on the solid phase and the other labelled with biotin. The serum is incubated simultaneously with the antibody-coated bead, the biotinylated antibody and
O M Steijger et al.
Journal of chromatography, 615(1), 97-110 (1993-05-19)
N-(4-Aminobutyl)-N-ethylisoluminol was used for labelling of carboxylic acids. The derivatization reaction was carried out with 1-hydroxybenzotriazole as pre-activator of the carboxylic acid function and N-ethyl-N'-(3-dimethylaminopropyl)carbodiimide as the coupling reagent. Optimum conditions for the derivatization were determined by using factorial design
R M Lequin et al.
Journal of immunoassay, 7(3), 169-179 (1986-01-01)
Chemiluminescent labels have been shown to be interesting alternatives to radioisotope labels. Disadvantages of the latter are preparation of e.g. labelled protein/peptides every four to six weeks, and problems with storage and disposal. Amino-Butyl-Ethyl-Isoluminol(ABEI) was attached to the alpha-amino function
Shulin Zhao et al.
Analytical biochemistry, 393(1), 105-110 (2009-06-23)
A microchip electrophoresis (MCE) method with chemiluminescence (CL) detection was developed for the determination of carnosine-related peptides, including carnosine, homocarnosine, and anserine, in biological samples. A simple integrated MCE-CL system was built to perform the assays. The highly sensitive CL

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