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94742

Sigma-Aldrich

Vanadyl ribonucleoside complexes solution

BioReagent, for molecular biology, 200 mM

Sinónimos:

Ribonucleoside vanadyl complexes

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.52

grade

for molecular biology

product line

BioReagent

form

liquid

concentration

200 mM

impurities

oxovanadium V

suitability

in accordance for ribonuclease inhibition test

shipped in

dry ice

storage temp.

−20°C

General description

Vanadyl ribonucleoside complexes are low molecular weight inhibitors of ribonucleases.
Vanadyl ribonucleoside complexes solution is an inhibitor for ribonuclease (RNase). Use of vanadyl ribonucleoside complexes exhibits protection from degradation by RNase in tissue, genomic, RNA and protein samples.

Application

Vanadyl ribonucleoside complexes (VDR) are transition state analogs that bind to active sites of many RNases, inhibiting their activity. Because the RNases are not covalently modified by the complexes, VDR must be used at every stage of RNA extraction and purification. However, VDR also inhibit RNA polymerases and in vitro translation and therefore must be removed from final preparation of RNA.
Vanadyl ribonucleoside complexes solution has been used:
  • as a component of polysome lysis buffer and fluorescence in situ hybridization (FISH) buffer
  • as a RNase inhibitor for the isolation of endogenous YTH domain-containing 2 protein (YTHDC2) from mouse testes

Related product

Referencia del producto
Descripción
Precios

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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