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Merck
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86R

Sigma-Aldrich

Leukocyte Alkaline Phosphatase Kit

based on naphthol AS-BI and fast red violet LB

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About This Item

UNSPSC Code:
12352106
eCl@ss:
42010105
NACRES:
NA.47

Quality Level

500

shelf life

Expiry date on the label.

IVD

for in vitro diagnostic use

dilution

(for histology)

application(s)

hematology
histology

shipped in

wet ice

storage temp.

2-8°C

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Application

Leukocyte Alkaline Phosphatase (LAP) kits are intended for the qualitative demonstration of alkaline phosphatase activity in white blood cells.
Peripheral blood or bone marrow preparations are fixed to a microscope slide. The film is then incubated in a mixture of naphthol AS-BI alkaline solution with fast red violet LB. The resulting insoluble diffuse, red dye deposit indicates sites of alkaline phosphatase activity.

Solo componentes del kit

Referencia del producto
Descripción
  • Citrate Solution (915) 50 mL
  • FRV-Alkaline Solution (862) 10 mL
  • Hematoxylin Solution, Gill No. 3 (kit only) 50 mL
  • Naphthol AS-BI Alkaline Solution (861) 10 mL
  • Sodium Nitrite Solution (914) 10 mL

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Met. Corr. 1 - Skin Irrit. 2 - STOT RE 2 Oral

target_organs

Kidney

Storage Class

8A - Combustible corrosive hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000283230
0000283230
SLCK4326
SLCH1193
SLCC4353

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Alessandro Polini et al.
PloS one, 6(10), e26211-e26211 (2011-10-25)
The development of a new family of implantable bioinspired materials is a focal point of bone tissue engineering. Implant surfaces that better mimic the natural bone extracellular matrix, a naturally nano-composite tissue, can stimulate stem cell differentiation towards osteogenic lineages
R Raz et al.
Proceedings of the National Academy of Sciences of the United States of America, 96(6), 2846-2851 (1999-03-17)
Propagation of mouse embryonic stem (ES) cells in vitro requires exogenous leukemia inhibitory factor (LIF) or related cytokines. Potential downstream effectors of the LIF signal in ES cells include kinases of the Src, Jak, and mitogen-activated protein families and the
Edgardo Rodríguez-Carballo et al.
PloS one, 9(7), e102032-e102032 (2014-07-10)
p38 MAPK activity plays an important role in several steps of the osteoblast lineage progression through activation of osteoblast-specific transcription factors and it is also essential for the acquisition of the osteoblast phenotype in early development. Although reports indicate p38
Salvatore Patanè et al.
Cancer research, 66(9), 4750-4757 (2006-05-03)
The MET oncogene was causally involved in the pathogenesis of a rare tumor, i.e., the papillary renal cell carcinoma, in which activating mutations, either germline or somatic, were identified. MET activating mutations are rarely found in other human tumors, whereas
Terence P Gade et al.
PloS one, 6(7), e22608-e22608 (2011-07-30)
The purpose of this study was to develop a paradigm for quantitative molecular imaging of bone cell activity. We hypothesized the feasibility of non-invasive imaging of the osteoblast enzyme alkaline phosphatase (ALP) using a small imaging molecule in combination with
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