84655
Schiff′s reagent for aldehydes
for microscopy
Sinónimos:
Fuchsin sulfurous acid
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About This Item
UNSPSC Code:
12352300
NACRES:
NA.21
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General description
Schiff′s reagent is used for the qualitative or quantitative assay of carbohydrates. Periodic acid-Schiff′s reagent (PAS) stain is broadly applicable in histology for visualizing glycogen, glycoproteins, mucins, and other polysaccharides in tissue and cells.
Application
Schiff′s reagent for aldehydes has been used:
- in microtiter PAS assay to quantify the amount of secreted mucin in Vibrio cholera infected water
- to stain the colonies of Clostridium perfringens in PGY agar plates to identify plasmalogen-deficient mutants
- in periodic acid-Schiff′s reagent assay to quantify carbohydrates in a microtiter plate format
signalword
Danger
hcodes
Hazard Classifications
Carc. 1B - Met. Corr. 1
Storage Class
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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David R Jackson et al.
ACS chemical biology, 16(1), 6-13 (2020-12-23)
Plasmalogens are vinyl ether-containing lipids produced by mammals and bacteria. The aerobic biosynthetic pathway in eukaryotes and bacteria is known, but the anaerobic pathway has remained a mystery. Here, we describe a two-gene operon (plasmalogen synthase, pls) responsible for plasmalogen
Vibrio cholerae Infection Induces Strain-Specific Modulation of the Zebrafish Intestinal Microbiome.
Paul Breen et al.
Infection and immunity, 89(9), e0015721-e0015721 (2021-06-02)
Zebrafish (Danio rerio) is an attractive model organism to use for an array of scientific studies, including host-microbe interactions. Zebrafish contain a core (i.e., consistently detected) intestinal microbiome consisting primarily of Proteobacteria. Furthermore, this core intestinal microbiome is plastic and
Paul Breen et al.
Infection and immunity, 89(9), e0015121-e0015121 (2021-06-08)
Zebrafish (Danio rerio) are an attractive model organism for a variety of scientific studies, including host-microbe interactions. The organism is particularly useful for the study of aquatic microbes that can colonize vertebrate hosts, including Vibrio cholerae, an intestinal pathogen. V.
Michelle Kilcoyne et al.
Analytical biochemistry, 416(1), 18-26 (2011-05-31)
Microtiter plate colorimetric assays are widely used for analysis of carbohydrates and glycoconjugates. However, mucins are often not easily detected, as they have low neutral sugar content. We have adapted and optimised the periodic acid-Schiff's reagent (PAS) staining for microtiter
Yixiang Zhong et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(3), e21379-e21379 (2021-02-11)
Traditionally cultured monolayers of primary human hepatocytes (PHHs) deteriorate within days and thereby become unsuitable for drug-related studies. PHH spheroids (3D PHHs) maintain liver functions for weeks, but are considerably more demanding. Recently, a chemical-based approach (5C PHHs) succeeded in
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