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Merck

75784

Sigma-Aldrich

Atto 565

BioReagent, suitable for fluorescence

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About This Item

Fórmula empírica (notación de Hill):
C31H31ClN2O9
Peso molecular:
611.04
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.32

product line

BioReagent

Quality Level

assay

≥90.0% (HPLC)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 562.0-568.0 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

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Application

Atto-Farbstoffe wurden entwickelt, um den höchsten Ansprüchen an Sensitivität zu genügen. Eine einmalige Kombination von Vorteilen macht sie zu bevorzugten Werkzeugen für alle Arten von Markierungen . Und einige Eigenschaften bieten besondere Vorzüge in der Einzelmoleküldetektion. Atto-Farbstoffe basieren auf rigiden Strukturen und zeigen keine cis-trans-isomerisierung. Letztere vermindert bei vielen anderen Fluorophoren die Helligkeit des Signals und führt zur Beeinflussbarkeit durch Umweltbedingungen, bspw. zu spektralen Verschiebungen bei der Konjugation.
Atto 565 hat eine molekulare Absorption von 120.000 und einen QY von 92% in Wasser (97% in Ethanol). Die Abklingzeit beträgt 3.4 ns.
Specific and stable fluorescence labeling of histidine-tagged proteins

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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We introduce a general approach for multicolor subdiffraction-resolution fluorescence imaging based on photoswitching of standard organic fluorophores. Photoswitching of ordinary fluorophores such as ATTO520, ATTO565, ATTO655, ATTO680, or ATTO700, i.e. the reversible transition from a fluorescent to a nonfluorescent state

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