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11557

Sigma-Aldrich

Ácido 2,2′-acino-bis(3-etilbenzotiazolina-6-sulfónico) diammonium salt

chromogenic, ≥98% (HPLC), powder or crystals

Sinónimos:

AzBTS-(NH4)2, Diamonio del 2,2′-acino-bis(3-etilbenzotiazolina-6-sulfonato)

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About This Item

Fórmula empírica (notación de Hill):
C18H24N6O6S4
Número de CAS:
30931-67-0
Peso molecular:
548.68
Beilstein/REAXYS Number:
7329461
EC Number:
250-396-6
MDL number:
MFCD00010404
UNSPSC Code:
12352204
PubChem Substance ID:
329749485
NACRES:
NA.83

product name

Ácido 2,2′-acino-bis(3-etilbenzotiazolina-6-sulfónico) diammonium salt, ≥98% (HPLC)

Quality Level

200

assay

≥98% (HPLC)

form

powder or crystals

impurities

≤6.0% water

solubility

H2O: 10 mg/mL, slightly hazy to clear

storage temp.

2-8°C

SMILES string

[NH4+].[NH4+].CCN1C(\Sc2cc(ccc12)S([O-])(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S([O-])(=O)=O

InChI

1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;

InChI key

OHDRQQURAXLVGJ-AXMZSLBLSA-N

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Application

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt has been used:
  • in the radical scavenging assay to test the antioxidant property of Pinus densiflora bark (PDB) extracts
  • to test the antioxidant capacity (AOC) of Aloe vera samples
  • as a substrate for streptavidin-horse radish peroxidase (HRPO) in enzyme-linked immunosorbent assay (ELISA)

El ácido 2,2′-acino-bis(3-etilbenzotiazolina-6-sulfónico es un sustrato de la peroxidasa adecuado para procedimientos ELISA. Este sustrato rinde un producto final soluble que es de color verde y puede leerse espectrofotométricamente a 405 nm. La reacción puede pararse con dodecilsulfatosódico (SDS) al 1 %. Recomendado para procedimientos ELISA (micropocillo), no recomendado para aplicaciones con membrana.

Other Notes

Chromogenic substrate for peroxidase; The ABTS-H2O2-peroxidase system in EIA

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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A Szutowicz et al.
Analytical biochemistry, 138(1), 86-94 (1984-04-01)
Monoamine oxidase is assayed in tissue by a colorimetric reaction using horse radish peroxidase and 2,2'-azinodi(ethylbenzthiazoline-6-sulfonic acid to measure H2O2 formed during oxidation of amines. The method has a coefficient of variation of approximately 2.5% and provides results comparable with
Prathik Roy et al.
Journal of hazardous materials, 243, 286-291 (2012-11-21)
We have developed a simple, colorimetric iron telluride (FeTe) nanorods (NRs) based system for the detection of mercury, mainly based on the cation exchange reaction between FeTe NRs and Hg(2+). FeTe NRs (length, 105 ± 21 nm) react with Hg(2+)
A Catarina Guedes et al.
Food chemistry, 138(1), 638-643 (2012-12-26)
A renewed interest in antioxidants has arisen in recent years; microalgae and cyanobacteria are potential sources thereof for use as food/feed ingredients. However, improved methods for comprehensive screening of antioxidant capacity specifically in intracellular extracts of marine microorganisms are required
Yi-Kai Chih et al.
Bioelectrochemistry (Amsterdam, Netherlands), 91, 44-51 (2013-02-19)
An 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS)-immobilized carbon nanotube (CNT) electrode was used to simultaneously detect dopamine (DA) and uric acid (UA) in the presence of ascorbic acid (AA) with differential pulse voltammetry. When ABTS was immobilized onto the CNT electrode in the
Jianwei Dong et al.
Journal of bioscience and bioengineering, 118(4), 396-399 (2014-04-20)
The tubers of Bletilla formosana were fermented with eight plant pathogen fungi, respectively, and antioxidant activities and total phenolic content (TPC) of the crude extracts of fermented products and non-fermented products were investigated. The antioxidant activities were evaluated in three
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