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Merck
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Documentos clave

11012002

Sigma-Aldrich

KYAE-1 cell line

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About This Item

UNSPSC Code:
41106514

biological source

human esophagus

packaging

tube of 5 μg 11012002-DNA-5UG
pkg of vial of cells 11012002-1VL

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial

products

P53 mutation c.455C>T

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

Cell Line Origin

Distal oesophageal adenocarcinoma,

Packaging

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11,12
D16S539: 11
D5S818: 10,13
D7S820: 11,12
THO1: 6
TPOX: 8
vWA: 14

Culture Medium

RPMI-1640:Hams F12 1:1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 3 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. There is a requirement to change the culture medium every 2-3 days.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number

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Visite la Librería de documentos

Donna E Hansel et al.
The American journal of surgical pathology, 29(3), 390-399 (2005-02-24)
Esophageal adenocarcinoma arises through well-defined precursor lesions (Barrett esophagus), although only a subset of these lesions advances to invasive adenocarcinoma. The lack of markers predicting progression in Barrett esophagus, typical presentation at advanced stage, and limitations of conventional chemotherapy result
T Kan et al.
Biochemical and biophysical research communications, 286(4), 792-801 (2001-08-25)
Human esophageal cancer cell lines and human esophageal cancer tissues were profiled on cDNA microarrays. In esophageal cancer cell lines, KYAE and OE-33 (adenocarcinomas) were distinguished from KYSE series (squamous cell carcinomas). Although SK-GT-4 and TE7 were derived from adenocarcinomas
Jurjen J Boonstra et al.
Journal of the National Cancer Institute, 102(4), 271-274 (2010-01-16)
For decades, hundreds of different human tumor type-specific cell lines have been used in experimental cancer research as models for their respective tumors. The veracity of experimental results for a specific tumor type relies on the correct derivation of the

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